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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 6 - Molecular Biology (D. Weigel)     Display Documents

ID: 706644.0, MPI für Entwicklungsbiologie / Abteilung 6 - Molecular Biology (D. Weigel)
A promiscuous intermediate underlies the evolution of LEAFY DNA binding specificity
Authors:Sayou, C.; Monniaux, M.; Nanao, M. H.; Moyroud, E.; Brockington, S. F.; Thevenon, E.; Chahtane, H.; Warthmann, N.; Melkonian, M.; Zhang, Y.; Wong, G. K.; Weigel, D.; Parcy, F.; Dumas, R.
Date of Publication (YYYY-MM-DD):2014-02-07
Title of Journal:Science
Journal Abbrev.:Science
Issue / Number:6171
Start Page:645
End Page:648
Sequence Number of Article:24436181
Review Status:Internal review
Audience:Experts Only
Abstract / Description:Transcription factors (TFs) are key players in evolution. Changes affecting their function can yield novel life forms but may also have deleterious effects. Consequently, gene duplication events that release one gene copy from selective pressure are thought to be the common mechanism by which TFs acquire new activities. Here, we show that LEAFY, a major regulator of flower development and cell division in land plants, underwent changes to its DNA binding specificity, even though plant genomes generally contain a single copy of the LEAFY gene. We examined how these changes occurred at the structural level and identify an intermediate LEAFY form in hornworts that appears to adopt all different specificities. This promiscuous intermediate could have smoothed the evolutionary transitions, thereby allowing LEAFY to evolve new binding specificities while remaining a single-copy gene.
Free Keywords:Amino Acid Sequence; Arabidopsis Proteins/chemistry/classification/genetics; DNA, Plant/*chemistry; DNA-Binding Proteins/*chemistry/classification/*genetics; Electrophoretic Mobility Shift Assay; *Evolution, Molecular; Gene Dosage; Molecular Sequence Data; Mutation; Phylogeny; Plant Proteins/*chemistry/classification/*genetics; Protein Binding/genetics; Protein Structure, Tertiary; Species Specificity; Transcription Factors/chemistry/classification/genetics
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilung 6 - Molekulare Biologie (Detlef Weigel)
Identifiers:ISSN:1095-9203 (Electronic) 0036-8075 (Linking) %R 10.1... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/pubmed/24436181 [ID No:2]
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