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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 6 - Molecular Biology (D. Weigel)     Display Documents



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ID: 706658.0, MPI für Entwicklungsbiologie / Abteilung 6 - Molecular Biology (D. Weigel)
New Arabidopsis advanced intercross recombinant inbred lines reveal female control of nonrandom mating
Authors:Fitz Gerald, J. N.; Carlson, A. L.; Smith, E.; Maloof, J. N.; Weigel, D.; Chory, J.; Borevitz, J. O.; Swanson, R. J.
Date of Publication (YYYY-MM-DD):2014-05
Title of Journal:Plant Physiology
Journal Abbrev.:Plant Physiol
Volume:165
Issue / Number:1
Start Page:175
End Page:185
Sequence Number of Article:24623850
Review Status:Internal review
Audience:Experts Only
Abstract / Description:Female control of nonrandom mating has never been genetically established, despite being linked to inbreeding depression and sexual selection. In order to map the loci that control female-mediated nonrandom mating, we constructed a new advanced intercross recombinant inbred line (RIL) population derived from a cross between Arabidopsis (Arabidopsis thaliana) accessions Vancouver (Van-0) and Columbia (Col-0) and mapped quantitative trait loci (QTLs) responsible for nonrandom mating and seed yield traits. We genotyped a population of 490 RILs. A subset of these lines was used to construct an expanded map of 1,061.4 centimorgans with an average interval of 6.7+/-5.3 centimorgans between markers. QTLs were then mapped for female- and male-mediated nonrandom mating and seed yield traits. To map the genetic loci responsible for female-mediated nonrandom mating and seed yield, we performed mixed pollinations with genetically marked Col-0 pollen and Van-0 pollen on RIL pistils. To map the loci responsible for male-mediated nonrandom mating and seed yield, we performed mixed pollinations with genetically marked Col-0 and RIL pollen on Van-0 pistils. Composite interval mapping of these data identified four QTLs that control female-mediated nonrandom mating and five QTLs that control female-mediated seed yield. We also identified four QTLs that control male-mediated nonrandom mating and three QTLs that control male-mediated seed yield. Epistasis analysis indicates that several of these loci interact. To our knowledge, the results of these experiments represent the first time female-mediated nonrandom mating has been genetically defined.
Free Keywords:Arabidopsis/*genetics; Chromosome Mapping; *Crosses, Genetic; Epistasis, Genetic; Genetic Markers; *Inbreeding; Inheritance Patterns/genetics; Phenotype; Quantitative Trait Loci/genetics; Quantitative Trait, Heritable; Recombination, Genetic/*genetics; Reproduction; Seeds/genetics/growth & development
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilung 6 - Molekulare Biologie (Detlef Weigel)
Identifiers:ISSN:1532-2548 (Electronic) 0032-0889 (Linking) %R 10.1... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/pubmed/24623850 [ID No:2]
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