Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 5 - Cell Biology (G. Jürgens)     Display Documents



  history
ID: 706961.0, MPI für Entwicklungsbiologie / Abteilung 5 - Cell Biology (G. Jürgens)
Cell type-specific transcriptome analysis in the early Arabidopsis thaliana embryo
Authors:Slane, D.; Kong, J.; Berendzen, K. W.; Kilian, J.; Henschen, A.; Kolb, M.; Schmid, M.; Harter, K.; Mayer, U.; De Smet, I.; Bayer, M.; Jurgens, G.
Date of Publication (YYYY-MM-DD):2014-12-15
Title of Journal:Development
Journal Abbrev.:Development
Volume:141
Issue / Number:24
Start Page:4831
End Page:4840
Sequence Number of Article:25411212
Review Status:Internal review
Audience:Experts Only
Abstract / Description:In multicellular organisms, cellular differences in gene activity are a prerequisite for differentiation and establishment of cell types. In order to study transcriptome profiles, specific cell types have to be isolated from a given tissue or even the whole organism. However, whole-transcriptome analysis of early embryos in flowering plants has been hampered by their size and inaccessibility. Here, we describe the purification of nuclear RNA from early stage Arabidopsis thaliana embryos using fluorescence-activated nuclear sorting (FANS) to generate expression profiles of early stages of the whole embryo, the proembryo and the suspensor. We validated our datasets of differentially expressed candidate genes by promoter-reporter gene fusions and in situ hybridization. Our study revealed that different classes of genes with respect to biological processes and molecular functions are preferentially expressed either in the proembryo or in the suspensor. This method can be used especially for tissues with a limited cell population and inaccessible tissue types. Furthermore, we provide a valuable resource for research on Arabidopsis early embryogenesis.
Free Keywords:Arabidopsis/*embryology/metabolism; Cell Nucleus/*chemistry; Cloning, Molecular; Gene Expression Profiling/*methods; Genotype; In Situ Hybridization; Microarray Analysis; Microscopy, Fluorescence; RNA, Nuclear/*isolation & purification; Real-Time Polymerase Chain Reaction; Seeds/*metabolism
External Publication Status:published
Document Type:Article
Communicated by:edocabt5
Affiliations:MPI für Entwicklungsbiologie/Abteilung 6 - Molekulare Biologie (Detlef Weigel)
External Affiliations:Department of Cell Biology, Max Planck Institute for Developmental Biology, Tubingen 72076, Germany Department of Developmental Genetics, Center for Plant Molecular Biology, University of Tubingen, Tubingen 72076, Germany. Department of Plant Physiology, Center for Plant Molecular Biology, University of Tubingen, Tubingen 72076, Germany. Department of Molecular Biology, Max Planck Institute for Developmental Biology, Tubingen 72076, Germany. Microscopy facility, Center for Plant Molecular Biology, University of Tubingen, Tubingen 72076, Germany. Department of Plant Systems Biology, VIB, Technologiepark 927, Ghent B-9052, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, Ghent B-9052, Belgium Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK. Department of Cell Biology, Max Planck Institute for Developmental Biology, Tubingen 72076, Germany Department of Developmental Genetics, Center for Plant Molecular Biology, University of Tubingen, Tubingen 72076, Germany gerd.juergens@zmbp.uni-tuebingen.de.
Identifiers:ISSN:1477-9129 (Electronic) 0950-1991 (Linking) %R 10.1... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/pubmed/25411212 [ID No:2]
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.