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          Institute: MPI für bioanorganische Chemie     Collection: MPI für bioanorganische Chemie     Display Documents



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ID: 708876.0, MPI für bioanorganische Chemie / MPI für bioanorganische Chemie
The Dark Recovery Rate in the Photocycle of the Bacterial Photoreceptor YtvA Is Affected by the Cellular Environment and by Hydration
Authors:Pennacchietti, Francesca; Abbruzzetti, Stefania; Losi, Aba; Mandalari, Carmen; Bedotti, Roberta; Viappiani, Cristiano; Zanacchi, Francesca C.; Diaspro, Alberto; Gärtner, Wolfgang
Language:English
Date of Publication (YYYY-MM-DD):2014
Title of Journal:PLoS ONE
Journal Abbrev.:Plos one
Volume:9
Issue / Number:9
Start Page:1
End Page:8
Sequence Number of Article:e107489
Review Status:Peer-review
Audience:Experts Only
Abstract / Description:We report thermal recovery kinetics of the lit state into the parental dark state, measured for the blue light-sensing photoreceptor YtvA inside overexpressing E. coli and B. subtilis bacterial cells, performed for the wild type and several mutated proteins. Recovery was followed as a recovery of the fluorescence, as this property is only found for the parental but not for the photochemically generated lit state. When cells were deposited onto a microscope glass plate, the observed thermal recovery rate in the photocycle was found ca. ten times faster in comparison to purified YtvA in solution. When the E. coli or B. subtilis colonies were soaked in an isotonic buffer, the dark relaxation became again much slower and was very similar to that observed for YtvA in solution. The observed effects show that rate constants can be tuned by the cellular environment through factors such as hydration.
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für bioanorganische Chemie
External Affiliations:[Pennacchietti,F.;Zanacchi,F.C.;Diaspro,A.;] Fdn.Ist.Italiano Tecnol., Genoa, Italy.
[Abbruzzetti,S.; Losi,A.; Mandalari,C.; Bedotti,R.; Viappiani,C.;] Univ Parma, Dipartimento Fis & Sci Terra, I-43100 Parma, Italy.
Identifiers:ISI:000341855900078 [ID No:1]
ISSN:1932-6203 [ID No:2]
DOI:10.1371/journal.pone.0107489 [ID No:3]
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