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          Institute: MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut)     Collection: Yearbook_2015     Display Documents



ID: 711862.0, MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut) / Yearbook_2015
Arterial hypertension in a murine model of sleep apnea: role of NADPH oxidase 2
Authors:Schulz, R.; Murzabekova, G.; Egemnazarov, B.; Kraut, S.; Eisele, H. J.; Dumitrascu, R.; Heitmann, J.; Seimetz, M.; Witzenrath, M.; Ghofrani, H. A.; Schermuly, R. T.; Grimminger, F.; Seeger, W.; Weissmann, N.
Date of Publication (YYYY-MM-DD):2014-02
Title of Journal:J Hypertens
Volume:32
Issue / Number:2
Start Page:300
End Page:305
Audience:Not Specified
Abstract / Description:OBJECTIVES: To investigate whether NADPH oxidase 2 (NOX2), a major source of reactive oxygen species (ROS), contributes to the emergence of arterial hypertension in a murine model of sleep apnea. BACKGROUND: Obstructive sleep apnea (OSA) is a risk factor for arterial hypertension and it is linked to oxidative stress. METHODS: C57BL/6J mice were exposed to chronic intermittent hypoxia (CIH) for 6 weeks (5 days/week, 8 h/day, alternating cycles of hypoxia and normoxia, each lasting 120 s, nadir FiO2: 7%). Blood pressure was monitored by telemetric catheters implanted into the abdominal aorta. Pharmacological inhibition of NOX by apocynin and NOX2-deficient mice were used to assess the role of NOX in CIH-induced arterial hypertension. NOX2 gene expression was measured by real-time PCR in different cardiovascular tissues. RESULTS: When compared with room air conditions, wild-type mice showed significant blood pressure elevations after exposure to CIH. This response was attenuated after treating animals with apocynin and in NOX2 (=gp91) knockout mice, whereas NOX2 was not upregulated in the heart, aorta, and femoral/carotid arteries of CIH mice. CONCLUSION: We suggest that the CIH-induced arterial hypertension is mediated by ROS derived from an activation of NOX2 within cells located outside the cardiovascular system.
Free Keywords:Acetophenones/pharmacology; Animals; Blood Pressure/physiology; Disease Models, Animal; Enzyme Inhibitors/pharmacology; Hypertension/*enzymology/*etiology/physiopathology; Male; Membrane Glycoproteins/antagonists & inhibitors/genetics/*physiology; Mice; Mice, Inbred C57BL; Mice, Knockout; NADPH Oxidase/antagonists & inhibitors/genetics/*physiology; Oxidative Stress; RNA, Messenger/genetics/metabolism; Reactive Oxygen Species/metabolism; Risk Factors; Sleep Apnea, Obstructive/*complications/*enzymology
External Publication Status:published
Document Type:Article
Communicated by:MPI für Herz- und Lungenforschung
Affiliations:MPI für physiologische und klinische Forschung
External Affiliations:%^ 1435822045
Identifiers:ISSN:1473-5598 (Electronic) 0263-6352 (Linking) %R 10.1097/HJH.0000000000000016
URL:http://www.ncbi.nlm.nih.gov/pubmed/24270180
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