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          Institute: MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut)     Collection: Yearbook_2015     Display Documents



ID: 711878.0, MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut) / Yearbook_2015
Doublecortin marks a new population of transiently amplifying muscle progenitor cells and is required for myofiber maturation during skeletal muscle regeneration
Authors:Ogawa, R.; Ma, Y.; Yamaguchi, M.; Ito, T.; Watanabe, Y.; Ohtani, T.; Murakami, S.; Uchida, S.; De Gaspari, P.; Uezumi, A.; Nakamura, M.; Miyagoe-Suzuki, Y.; Tsujikawa, K.; Hashimoto, N.; Braun, T.; Tanaka, T.; Takeda, S.; Yamamoto, H.; Fukada, S.
Date of Publication (YYYY-MM-DD):2015-01-01
Title of Journal:Development
Volume:142
Issue / Number:1
Start Page:51
End Page:61
Audience:Not Specified
Abstract / Description:Muscle satellite cells are indispensable for muscle regeneration, but the functional diversity of their daughter cells is unknown. Here, we show that many Pax7(+)MyoD(-) cells locate both beneath and outside the basal lamina during myofiber maturation. A large majority of these Pax7(+)MyoD(-) cells are not self-renewed satellite cells, but have different potentials for both proliferation and differentiation from Pax7(+)MyoD(+) myoblasts (classical daughter cells), and are specifically marked by expression of the doublecortin (Dcx) gene. Transplantation and lineage-tracing experiments demonstrated that Dcx-expressing cells originate from quiescent satellite cells and that the microenvironment induces Dcx in myoblasts. Expression of Dcx seems to be necessary for myofiber maturation because Dcx-deficient mice exhibited impaired myofiber maturation resulting from a decrease in the number of myonuclei. Furthermore, in vitro and in vivo studies suggest that one function of Dcx in myogenic cells is acceleration of cell motility. These results indicate that Dcx is a new marker for the Pax7(+)MyoD(-) subpopulation, which contributes to myofiber maturation during muscle regeneration.
Free Keywords:Animals; Cardiotoxins/administration & dosage; *Cell Differentiation; Cell Movement; Cellular Microenvironment; Mice, Inbred C57BL; Mice, Knockout; Microtubule-Associated Proteins/deficiency/*metabolism; Muscle Fibers, Skeletal/*cytology/*physiology; MyoD Protein/metabolism; Myoblasts/cytology/metabolism; Neuropeptides/deficiency/*metabolism; PAX7 Transcription Factor/metabolism; Regeneration/*physiology; Satellite Cells, Skeletal Muscle/cytology; Stem Cells/*cytology/metabolism
External Publication Status:published
Document Type:Article
Communicated by:MPI für Herz- und Lungenforschung
Affiliations:MPI für physiologische und klinische Forschung
External Affiliations:Institute of Cardiovascular Regeneration, Centre for Molecular Medicine, Goethe University Frankfurt, Theodor-Stern-Kai 7, Frankfurt am Main 60590, Germany. Max Planck Institute for Heart and Lung Research, Ludwigstrasse 43, Bad Nauheim 61231, Germany. Division for Therapies Against Intractable Diseases, Institute for Comprehensive Medical Science, Fujita Health University, 1-98 Dengakugakubo, Kutsukake, Toyoake, Aichi 470-1192, Japan. Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-1 Ogawa-higashi, Kodaira, Tokyo 187-8502, Japan. Department of Regenerative Medicine, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, 35 Gengo, Morioka, Oobu, Aichi 474-8522, Japan. Department of Developmental Medical Sciences, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. Laboratory of Molecular and Cellular Physiology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan fukada@phs.osaka-u.ac.jp. %^ 1435822261
Identifiers:ISSN:1477-9129 (Electronic) 0950-1991 (Linking) %R 10.1242/dev.112557
URL:http://www.ncbi.nlm.nih.gov/pubmed/25480916
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