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          Institute: MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut)     Collection: Yearbook_2015     Display Documents



  history
ID: 711902.0, MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut) / Yearbook_2015
Sham surgery and inter-individual heterogeneity are major determinants of monocyte subset kinetics in a mouse model of myocardial infarction
Authors:Hoffmann, J.; Ospelt, M.; Troidl, C.; Voss, S.; Liebetrau, C.; Kim, W. K.; Rolf, A.; Wietelmann, A.; Braun, T.; Troidl, K.; Sadayappan, S.; Barefield, D.; Hamm, C.; Nef, H.; Mollmann, H.
Date of Publication (YYYY-MM-DD):2014
Title of Journal:PLoS ONE
Volume:9
Issue / Number:6
Start Page:e98456
Audience:Not Specified
Abstract / Description:AIMS: Mouse models of myocardial infarction (MI) are commonly used to explore the pathophysiological role of the monocytic response in myocardial injury and to develop translational strategies. However, no study thus far has examined the potential impact of inter-individual variability and sham surgical procedures on monocyte subset kinetics after experimental MI in mice. Our goal was to investigate determinants of systemic myeloid cell subset shifts in C57BL/6 mice following MI by developing a protocol for sequential extensive flow cytometry (FCM). METHODS AND RESULTS: Following cross-sectional multiplex FCM analysis we provide for the first time a detailed description of absolute quantities, relative subset composition, and biological variability of circulating classical, intermediate, and non-classical monocyte subsets in C57BL/6 mice. By using intra-individual longitudinal measurements after MI induction, a time course of classical and non-classical monocytosis was recorded. This approach disclosed a significant reduction of monocyte subset dispersion across all investigated time points following MI. We found that in the current invasive model of chronic MI the global pattern of systemic monocyte kinetics is mainly determined by a nonspecific inflammatory response to sham surgery and not by the extent of myocardial injury. CONCLUSIONS: Application of sequential multiplexed FCM may help to reduce the impact of biological variability in C57BL/6 mice. Furthermore, the confounding influence of sham surgical procedures should always be considered when measuring monocyte subset kinetics in a murine model of MI.
Free Keywords:Animals; Cross-Sectional Studies; Flow Cytometry; Immunohistochemistry; Kinetics; Male; Mice; Mice, Inbred C57BL; Monocytes/*metabolism; Myocardial Infarction/*surgery
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:MPI für Herz- und Lungenforschung
Affiliations:MPI für physiologische und klinische Forschung
External Affiliations:Department of Cardiology, Kerckhoff Heart and Thorax Center, Bad Nauheim, Germany; Department of Cardiology, University of Giessen, Medical Clinic I, Giessen, Germany. Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany. Department of Cell and Molecular Physiology, Health Sciences Division, Loyola University Chicago, Maywood, Illinois, United States of America. %^ 1435822116
Identifiers:ISSN:1932-6203 (Electronic) 1932-6203 (Linking) %R 10.1371/journal.pone.0098456
URL:http://www.ncbi.nlm.nih.gov/pubmed/24893162
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