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          Institute: MPI für bioanorganische Chemie     Collection: MPI für bioanorganische Chemie     Display Documents



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ID: 712830.0, MPI für bioanorganische Chemie / MPI für bioanorganische Chemie
Spectroscopic Investigations of [FeFe] Hydrogenase Maturated with [Fe-57(2)(adt)(CN)(2)(CO)(4)](2-)
Authors:Gilbert-Wilson, Ryan; Siebel, Judith F.; Adamska-Venkatesh, Agnieszka; Pham, Cindy C.; Reijerse, Edward J.; Wang, Hongxin; Cramer, Stephen P.; Lubitz, Wolfgang; Rauchfuss, Thomas B.
Language:English
Date of Publication (YYYY-MM-DD):2015
Title of Journal:Journal of the American Chemical Society
Journal Abbrev.:J.Am.Chem.Soc.
Volume:137
Issue / Number:28
Start Page:8998
End Page:9005
Review Status:Internal review
Audience:Experts Only
Abstract / Description:The preparation and spectroscopic characterization of a CO-inhibited [FeFe] hydrogenase with a selectively Fe-57-labeled binuclear subsite is described. The precursor [Fe-57(2)(adt)(CN)(2)(CO)(4)](2-) was synthesized from the Fe-57 metal, S-8, CO, (NEt4)CN, NH4Cl, and CH2O. (Et4N)(2)[Fe-57(2)(adt)(CN)(2)(CO)(4)] was then used for the maturation of the [FeFe] hydrogenase HydA1 from Chlamydomonas reinhardtii, to yield the enzyme selectively labeled at the [2Fe](H) subcluster. Complementary Fe-57 enrichment of the [4Fe-4S](H) cluster was realized by reconstitution with (FeCl3)-Fe-57 and Na2S. The H-ox-CO state of [2(57)Fe](H) and [4(57)Fe-4S](H) HydAl was characterized by Mossbauer, HYSCORE, ENDOR, and nuclear resonance vibrational spectroscopy.
Comment of the Author/Creator:Date: 2015, JUL 22 2015
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:N. N.
Affiliations:MPI für bioanorganische Chemie
External Affiliations:[Gilbert-Wilson,R.; Rauchfuss,T.B.] Univ Illinois, Sch Chem Sci, Urbana, IL 61801 USA.
[Pham,C.C.; Wang,H.; Cramer,S.] Univ Calif Davis, Dept Chem, Davis, CA 95616 USA.
Identifiers:ISI:000358556200026 [ID No:1]
ISSN:0002-7863 [ID No:2]
DOI:10.1021/jacs.5b03270 [ID No:3]
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