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          Institute: MPI für molekulare Biomedizin     Collection: Jahrbuch 2016 (publ. 2015, arch)     Display Documents



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ID: 717250.0, MPI für molekulare Biomedizin / Jahrbuch 2016 (publ. 2015, arch)
Intubation-based anesthesia for long-term time-lapse imaging of adult zebrafish
Authors:Xu, C.; Volkery, S.; Siekmann, A. F.
Date of Publication (YYYY-MM-DD):2015-12
Title of Journal:Nat Protoc
Volume:10
Issue / Number:12
Start Page:2064
End Page:2073
Review Status:Internal review
Audience:Not Specified
Abstract / Description:Zebrafish possess the remarkable ability to regenerate a vast variety of tissues, even as adults. However, direct imaging of regenerative processes in adult zebrafish remains challenging because of the lack of suitable anesthesia protocols. Here we present a description of an intubation-based anesthesia procedure that we developed to enable us to image regenerating zebrafish fins and which can be used to continuously anesthetize adult zebrafish for up to 2 d. Fish are immobilized in an imaging chamber followed by oral intubation. Subsequent delivery of anesthetic-containing water is achieved via a peristaltic pump. The setup of the system will take approximately 90 min for two adult zebrafish, and it requires only a little previous experience of working with zebrafish. Our protocol will enable the imaging of regenerative processes in the fin and other tissues, and the investigation of processes that require long-term anesthesia, such as immune responses and surgical procedures.
External Publication Status:published
Document Type:Article
Communicated by:Keuker
Affiliations:MPI für molekulare Biomedizin
Identifiers:ISSN:1750-2799 (Electronic) 1750-2799 (Linking) %R 10.1... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/pubmed/26584446 [ID No:2]
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