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          Institute: MPI für molekulare Biomedizin     Collection: Jahrbuch 2016 (publ. 2015, arch)     Display Documents



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ID: 717269.0, MPI für molekulare Biomedizin / Jahrbuch 2016 (publ. 2015, arch)
Beta-catenin-dependent control of positional information along the AP body axis in planarians involves a teashirt family member
Authors:Reuter, H.; Marz, M.; Vogg, M. C.; Eccles, D.; Grifol-Boldu, L.; Wehner, D.; Owlarn, S.; Adell, T.; Weidinger, G.; Bartscherer, K.
Date of Publication (YYYY-MM-DD):2015-01-13
Title of Journal:Cell Rep
Volume:10
Issue / Number:2
Start Page:253
End Page:265
Review Status:Internal review
Audience:Not Specified
Abstract / Description:Wnt/beta-catenin signaling regulates tissue homeostasis and regeneration in metazoans. In planarians-flatworms with high regenerative potential-Wnt ligands are thought to control tissue polarity by shaping a beta-catenin activity gradient along the anterior-posterior axis, yet the downstream mechanisms are poorly understood. We performed an RNA sequencing (RNA-seq)-based screen and identified hundreds of beta-catenin-dependent transcripts, of which several were expressed in muscle tissue and stem cells in a graded fashion. In particular, a teashirt (tsh) ortholog was induced in a beta-catenin-dependent manner during regeneration in planarians and zebrafish, and RNAi resulted in two-headed planarians. Strikingly, intact planarians depleted of tsh induced anterior markers and slowly transformed their tail into a head, reminiscent of beta-catenin RNAi phenotypes. Given that beta-catenin RNAi enhanced the formation of muscle cells expressing anterior determinants in tail regions, our study suggests that this pathway controls tissue polarity through regulating the identity of differentiating cells during homeostasis and regeneration.
Free Keywords:Animals; Base Sequence; Body Patterning/*physiology; Collagen/metabolism; Cytoskeletal Proteins/antagonists & inhibitors/genetics/metabolism; Helminth Proteins/antagonists & inhibitors/genetics/*metabolism; Molecular Sequence Data; Planarians; RNA Interference; RNA, Double-Stranded/metabolism; Regeneration; Repressor Proteins/antagonists & inhibitors/genetics/*metabolism; Stem Cells/cytology/metabolism; Transcriptome; Wnt Proteins/antagonists & inhibitors/metabolism; Wnt Signaling Pathway; Zebrafish; beta Catenin/antagonists & inhibitors/genetics/*metabolism
External Publication Status:published
Document Type:Article
Communicated by:Keuker
Affiliations:MPI für molekulare Biomedizin
External Affiliations:Max Planck Research Group for Stem Cells and Regeneration, Max Planck Institute for Molecular Biomedicine, Von-Esmarch-Strasse 54, 48149 Munster, Germany; Medical Faculty, University of Munster, Albert-Schweitzer-Campus 1, 48149 Munster, Germany. Malaghan Institute of Medical Research, Victoria University, Kelburn Parade, Wellington 6242, New Zealand. Max Planck Research Group for Stem Cells and Regeneration, Max Planck Institute for Molecular Biomedicine, Von-Esmarch-Strasse 54, 48149 Munster, Germany; Department of Genetics and Institute of Biomedicine, University of Barcelona, 08028 Barcelona, Catalonia, Spain. Institute for Biochemistry and Molecular Biology, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany. Department of Genetics and Institute of Biomedicine, University of Barcelona, 08028 Barcelona, Catalonia, Spain. Max Planck Research Group for Stem Cells and Regeneration, Max Planck Institute for Molecular Biomedicine, Von-Esmarch-Strasse 54, 48149 Munster, Germany; Medical Faculty, University of Munster, Albert-Schweitzer-Campus 1, 48149 Munster, Germany. Electronic address: kerstin.bartscherer@mpi-muenster.mpg.de.
Identifiers:ISSN:2211-1247 (Electronic) %R 10.1016/j.celrep.2014.12... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/pubmed/25558068 [ID No:2]
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