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          Institute: MPI für molekulare Biomedizin     Collection: Jahrbuch 2016 (publ. 2015, arch)     Display Documents



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ID: 717276.0, MPI für molekulare Biomedizin / Jahrbuch 2016 (publ. 2015, arch)
Human pluripotent stem cell-derived cardiomyocytes: Genome-wide expression profiling of long-term in vitro maturation in comparison to human heart tissue
Authors:Piccini, I.; Rao, J.; Seebohm, G.; Greber, B.
Date of Publication (YYYY-MM-DD):2015-06
Title of Journal:Genom Data
Volume:4
Start Page:69
End Page:72
Review Status:Internal review
Audience:Not Specified
Abstract / Description:Cardiomyocyte-like cells (CMs) derived from human pluripotent stem cells (hPSCs) present a valuable model for human disease modeling, studying early human development and, potentially, developing cell therapeutic approaches. However, the specification of early hPSC-derived CMs into defined cardiac subtypes such as atrial and ventricular cells is not well understood and, thus, poorly controlled. Moreover, the maturation status of hPSC-CMs is not well defined, yet it is known that these cells undergo at least some degree of maturation upon longer term in vitro culture. To gain insight into this process, and to assess their developmental status, we have recently generated a data set of hPSC-CMs monitoring global changes in gene expression upon long term maintenance in vitro, in comparison to human atrial and ventricular heart samples (GEO accession number GEO: GSE64189). These data present a rich resource for evaluating the maturation status of hPSC-CMs, for identifying suitable markers for subtype-specific gene expression, as well as for the generation of functional hypotheses. Here, we provide additional details and quality checks of this data set, and exemplify how it can be used to identify maturation-associated as well as cardiac subtype-specific markers.
Free Keywords:Cardiac differentiation; Cardiomyocytes; Human heart; Human pluripotent stem cells; In-vitro maturation
External Publication Status:published
Document Type:Article
Communicated by:Keuker
Affiliations:MPI für molekulare Biomedizin
External Affiliations:Human Stem Cell Pluripotency Laboratory, Max Planck Institute for Molecular Biomedicine, Munster, Germany ; Chemical Genomics Centre of the Max Planck Society, Dortmund, Germany.
Identifiers:ISSN:2213-5960 (Electronic) 2213-5960 (Linking) %R 10.1... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/pubmed/26484180 [ID No:2]
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