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          Institute: MPI für molekulare Biomedizin     Collection: Jahrbuch 2016 (publ. 2015, arch)     Display Documents



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ID: 717302.0, MPI für molekulare Biomedizin / Jahrbuch 2016 (publ. 2015, arch)
Reactivation of the inactive X chromosome and post-transcriptional reprogramming of Xist in iPSCs
Authors:Kim, J. S.; Choi, H. W.; Arauzo-Bravo, M. J.; Scholer, H. R.; Do, J. T.
Date of Publication (YYYY-MM-DD):2015-01-01
Title of Journal:J Cell Sci
Volume:128
Issue / Number:1
Start Page:81
End Page:87
Review Status:Internal review
Audience:Not Specified
Abstract / Description:Direct reprogramming of somatic cells to pluripotent stem cells entails the obliteration of somatic cell memory and the reestablishment of epigenetic events. Induced pluripotent stem cells (iPSCs) have been created by reprogramming somatic cells through the transduction of reprogramming factors. During cell reprogramming, female somatic cells must overcome at least one more barrier than male somatic cells in order to enter a pluripotent state, as they must reactivate an inactive X chromosome (Xi). In this study, we investigated whether the sex of somatic cells affects reprogramming efficiency, differentiation potential and the post-transcriptional processing of Xist RNA after reprogramming. There were no differences between male and female iPSCs with respect to reprogramming efficiency or their differentiation potential in vivo. However, reactivating Xi took longer than reactivating pluripotency-related genes. We also found that direct reprogramming leads to gender-appropriate post-transcriptional reprogramming - like male embryonic stem cells (ESCs), male iPSCs expressed only the long Xist isoform, whereas female iPSCs, like female ESCs, expressed both the long and short isoforms.
Free Keywords:Animals; *Cellular Reprogramming; Female; Humans; Induced Pluripotent Stem Cells/cytology/*metabolism; Male; Mice; Mice, Knockout; RNA, Long Noncoding/*biosynthesis/genetics; *Sex Characteristics; X Chromosome/genetics/*metabolism; *X Chromosome Inactivation; Oct4; Pluripotency; Reprogramming; Xist isoform; iPSC
External Publication Status:published
Document Type:Article
Communicated by:Keuker
Affiliations:MPI für molekulare Biomedizin
External Affiliations:Group of Computational Biology and Systems Biomedicine, Biodonostia Health Research Institute, San Sebastian 20014, Spain IKERBASQUE, Basque Foundation for Science, Bilbao 48013, Spain. Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Rontgenstrasse 20, Munster 48149, Germany. Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 133-702, Republic of Korea dojt@konkuk.ac.kr.
Identifiers:ISSN:1477-9137 (Electronic) 0021-9533 (Linking) %R 10.1... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/pubmed/25380819 [ID No:2]
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