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          Institute: MPI für molekulare Biomedizin     Collection: Jahrbuch 2016 (publ. 2015, arch)     Display Documents



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ID: 717303.0, MPI für molekulare Biomedizin / Jahrbuch 2016 (publ. 2015, arch)
Oct4-induced oligodendrocyte progenitor cells enhance functional recovery in spinal cord injury model
Authors:Kim, J. B.; Lee, H.; Arauzo-Bravo, M. J.; Hwang, K.; Nam, D.; Park, M. R.; Zaehres, H.; Park, K. I.; Lee, S. J.
Date of Publication (YYYY-MM-DD):2015-12-02
Title of Journal:EMBO J
Volume:34
Issue / Number:23
Start Page:2971
End Page:2983
Review Status:Internal review
Audience:Not Specified
Abstract / Description:The generation of patient-specific oligodendrocyte progenitor cells (OPCs) holds great potential as an expandable cell source for cell replacement therapy as well as drug screening in spinal cord injury or demyelinating diseases. Here, we demonstrate that induced OPCs (iOPCs) can be directly derived from adult mouse fibroblasts by Oct4-mediated direct reprogramming, using anchorage-independent growth to ensure high purity. Homogeneous iOPCs exhibit typical small-bipolar morphology, maintain their self-renewal capacity and OPC marker expression for more than 31 passages, share high similarity in the global gene expression profile to wild-type OPCs, and give rise to mature oligodendrocytes and astrocytes in vitro and in vivo. Notably, transplanted iOPCs contribute to functional recovery in a spinal cord injury (SCI) model without tumor formation. This study provides a simple strategy to generate functional self-renewing iOPCs and yields insights for the in-depth study of demyelination and regenerative medicine.
Free Keywords:Oct4; direct conversion; myelination; oligodendrocyte progenitor cell; self-renewal
External Publication Status:published
Document Type:Article
Communicated by:Keuker
Affiliations:MPI für molekulare Biomedizin
External Affiliations:Hans Scholer Stem Cell Research Center (HSSCRC), School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan, South Korea Max Planck Partner Group-Molecular Biomedicine Laboratory (MPPG-MBL), UNIST, Ulsan, South Korea. Group of Computational Biology and Bioinformatics, Biodonostia Health Research Institute, San Sebastian, Spain IKERBASQUE, Basque Foundation for Science, Bilbao, Spain. Department of Pediatrics and BK21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul, South Korea. Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Munster, Germany.
Identifiers:ISSN:1460-2075 (Electronic) 0261-4189 (Linking) %R 10.1... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/pubmed/26497893 [ID No:2]
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