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          Institute: MPI für molekulare Biomedizin     Collection: Jahrbuch 2016 (publ. 2015, arch)     Display Documents



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ID: 717304.0, MPI für molekulare Biomedizin / Jahrbuch 2016 (publ. 2015, arch)
Generation of integration-free induced hepatocyte-like cells from mouse fibroblasts
Authors:Kim, J.; Kim, K. P.; Lim, K. T.; Lee, S. C.; Yoon, J.; Song, G.; Hwang, S. I.; Scholer, H. R.; Cantz, T.; Han, D. W.
Date of Publication (YYYY-MM-DD):2015
Title of Journal:Sci Rep
Volume:5
Start Page:15706
Review Status:Internal review
Audience:Not Specified
Abstract / Description:The ability to generate integration-free induced hepatocyte-like cells (iHeps) from somatic fibroblasts has the potential to advance their clinical application. Here, we have generated integration-free, functional, and expandable iHeps from mouse somatic fibroblasts. To elicit this direct conversion, we took advantage of an oriP/EBNA1-based episomal system to deliver a set of transcription factors, Gata4, Hnf1a, and Foxa3, to the fibroblasts. The established iHeps exhibit similar morphology, marker expression, and functional properties to primary hepatocytes. Furthermore, integration-free iHeps prolong the survival of fumarylacetoacetate-hydrolase-deficient (Fah(-/-)) mice after cell transplantation. Our study provides a novel concept for generating functional and expandable iHeps using a non-viral, non-integrating, plasmid-based system that could facilitate their pharmaceutical and biomedical application.
External Publication Status:published
Document Type:Article
Communicated by:Keuker
Affiliations:MPI für molekulare Biomedizin
External Affiliations:Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Rontgenstrasse 20, 48149 Munster, Germany. REBIRTH Cluster of Excellence, Hannover Medical School, Hannover 30625, Germany. University of Munster, Medical Faculty, Domagkstrasse 3, 48149 Munster, Germany. KU Open-Innovation Center, Institute of Biomedical Science &Technology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea. %W NLM %G eng
Identifiers:ISSN:2045-2322 %R 10.1038/srep15706 [ID No:1]
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