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          Institute: MPI für molekulare Biomedizin     Collection: Jahrbuch 2016 (publ. 2015, arch)     Display Documents



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ID: 717312.0, MPI für molekulare Biomedizin / Jahrbuch 2016 (publ. 2015, arch)
Chemokine-guided angiogenesis directs coronary vasculature formation in zebrafish
Authors:Harrison, M. R.; Bussmann, J.; Huang, Y.; Zhao, L.; Osorio, A.; Burns, C. G.; Burns, C. E.; Sucov, H. M.; Siekmann, A. F.; Lien, C. L.
Date of Publication (YYYY-MM-DD):2015-05-26
Title of Journal:Dev Cell
Volume:33
Issue / Number:4
Start Page:442
End Page:454
Review Status:Internal review
Audience:Not Specified
Abstract / Description:Interruption of the coronary blood supply severely impairs heart function with often fatal consequences for patients. However, the formation and maturation of these coronary vessels is not fully understood. Here we provide a detailed analysis of coronary vessel development in zebrafish. We observe that coronary vessels form in zebrafish by angiogenic sprouting of arterial cells derived from the endocardium at the atrioventricular canal. Endothelial cells express the CXC-motif chemokine receptor Cxcr4a and migrate to vascularize the ventricle under the guidance of the myocardium-expressed ligand Cxcl12b. cxcr4a mutant zebrafish fail to form a vascular network, whereas ectopic expression of Cxcl12b ligand induces coronary vessel formation. Importantly, cxcr4a mutant zebrafish fail to undergo heart regeneration following injury. Our results suggest that chemokine signaling has an essential role in coronary vessel formation by directing migration of endocardium-derived endothelial cells. Poorly developed vasculature in cxcr4a mutants likely underlies decreased regenerative potential in adults.
Free Keywords:Angiography; Animals; Animals, Genetically Modified/genetics/*growth & development/metabolism; Chemokines, CXC/*metabolism; Coronary Vessels/embryology/*growth & development; Embryo, Nonmammalian/cytology/*metabolism; Endothelium, Vascular/cytology/metabolism; Gene Expression Regulation, Developmental; Heart/physiology; Microscopy, Confocal; *Neovascularization, Physiologic; Organogenesis/physiology; Receptors, CXCR4/*metabolism; Regeneration/physiology; Signal Transduction; Zebrafish/genetics/*growth & development/metabolism; Zebrafish Proteins/*metabolism
External Publication Status:published
Document Type:Article
Communicated by:Keuker
Affiliations:MPI für molekulare Biomedizin
External Affiliations:Max Planck Institute for Molecular Biomedicine, Muenster 48149, Germany. Cardiovascular Research Center, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA. Broad CIRM Center and Department of Stem Cell Biology and Regenerative Medicine, University of Southern California, Los Angeles, CA 90033, USA. Heart Institute, The Saban Research Institute of Children's Hospital Los Angeles, Los Angeles, CA 90027, USA; Program of Developmental Biology and Regenerative Medicine, The Saban Research Institute of Children's Hospital Los Angeles, Los Angeles, CA 90027, USA; Department of Surgery, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA; Department of Biochemistry & Molecular Biology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA. Electronic address: clien@chla.usc.edu.
Identifiers:ISSN:1878-1551 (Electronic) 1534-5807 (Linking) %R 10.1... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/pubmed/26017769 [ID No:2]
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