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          Institute: MPI für molekulare Biomedizin     Collection: Jahrbuch 2016 (publ. 2015, arch)     Display Documents



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ID: 717322.0, MPI für molekulare Biomedizin / Jahrbuch 2016 (publ. 2015, arch)
Erythroid differentiation of human induced pluripotent stem cells is independent of donor cell type of origin
Authors:Dorn, I.; Klich, K.; Arauzo-Bravo, M. J.; Radstaak, M.; Santourlidis, S.; Ghanjati, F.; Radke, T. F.; Psathaki, O. E.; Hargus, G.; Kramer, J.; Einhaus, M.; Kim, J. B.; Kogler, G.; Wernet, P.; Scholer, H. R.; Schlenke, P.; Zaehres, H.
Date of Publication (YYYY-MM-DD):2015-01
Title of Journal:Haematologica
Volume:100
Issue / Number:1
Start Page:32
End Page:41
Review Status:Internal review
Audience:Not Specified
Abstract / Description:Epigenetic memory in induced pluripotent stem cells, which is related to the somatic cell type of origin of the stem cells, might lead to variations in the differentiation capacities of the pluripotent stem cells. In this context, induced pluripotent stem cells from human CD34(+) hematopoietic stem cells might be more suitable for hematopoietic differentiation than the commonly used fibroblast-derived induced pluripotent stem cells. To investigate the influence of an epigenetic memory on the ex vivo expansion of induced pluripotent stem cells into erythroid cells, we compared induced pluripotent stem cells from human neural stem cells and human cord blood-derived CD34(+) hematopoietic stem cells and evaluated their potential for differentiation into hematopoietic progenitor and mature red blood cells. Although genome-wide DNA methylation profiling at all promoter regions demonstrates that the epigenetic memory of induced pluripotent stem cells is influenced by the somatic cell type of origin of the stem cells, we found a similar hematopoietic induction potential and erythroid differentiation pattern of induced pluripotent stem cells of different somatic cell origin. All human induced pluripotent stem cell lines showed terminal maturation into normoblasts and enucleated reticulocytes, producing predominantly fetal hemoglobin. Differences were only observed in the growth rate of erythroid cells, which was slightly higher in the induced pluripotent stem cells derived from CD34(+) hematopoietic stem cells. More detailed methylation analysis of the hematopoietic and erythroid promoters identified similar CpG methylation levels in the induced pluripotent stem cell lines derived from CD34(+) cells and those derived from neural stem cells, which confirms their comparable erythroid differentiation potential.
Free Keywords:Biomarkers/metabolism; *Cell Differentiation; DNA Methylation; Epigenomics; Erythroid Cells/*cytology/metabolism; Fetal Blood/*cytology/metabolism; Fibroblasts/cytology/metabolism; Gene Expression Profiling; Hematopoietic Stem Cells/*cytology/metabolism; Humans; Induced Pluripotent Stem Cells/*cytology/metabolism; Neural Stem Cells/*cytology/metabolism; RNA, Messenger/genetics; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction
External Publication Status:published
Document Type:Article
Communicated by:Keuker
Affiliations:MPI für molekulare Biomedizin
External Affiliations:Max Planck Institute for Molecular Biomedicine, Munster, Germany Institute for Transfusion Medicine and Transplantation Immunology, University Hospital Munster, Germany. Group of Computational Biology and Systems Biomedicine, Biodonostia Health Research Institute, San Sebastian, Spain IKERBASQUE, Basque Foundation for Science, Bilbao, Spain. Max Planck Institute for Molecular Biomedicine, Munster, Germany. Institute for Transplantation Diagnostics and Cell Therapeutics, Heinrich-Heine-University Dusseldorf, Germany. Max Planck Institute for Molecular Biomedicine, Munster, Germany Institute for Neuropathology, University Hospital Munster, Germany. Medical Department I, University of Lubeck, Germany LADR GmbH, Geesthacht, Germany. LADR GmbH, Geesthacht, Germany. UNIST, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea. Max Planck Institute for Molecular Biomedicine, Munster, Germany Faculty of Medicine, University of Munster, Germany. Institute for Transfusion Medicine and Transplantation Immunology, University Hospital Munster, Germany Clinics for Blood Group Serology and Transfusion Medicine, Medical University Graz, Austria. Max Planck Institute for Molecular Biomedicine, Munster, Germany isabel.dorn@mpi-muenster.mpg.de holm.zaehres@mpi-muenster.mpg.de.
Identifiers:ISSN:1592-8721 (Electronic) 0390-6078 (Linking) %R 10.3... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/pubmed/25326431 [ID No:2]
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