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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2015 (arch)     Display Documents



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ID: 717957.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2015 (arch)
Single-molecule imaging of cytoplasmic dynein in vivo.
Authors:Ananthanarayanan, Vaishnavi; Tolic, Iva M.
Date of Publication (YYYY-MM-DD):2015
Title of Journal:Methods in Cell Biology
Volume:125
Start Page:1
End Page:12
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:While early fluorescence microscopy experiments employing fluorescent probes afforded snapshots of the cell, the power of live-cell microscopy is required to understand complex dynamics in biological processes. The first successful cloning of green fluorescent protein in the 1990s paved the way for development of approaches that we now utilize for visualization in a living cell. In this chapter, we discuss a technique to observe fluorescently tagged single molecules in fission yeast. With a few simple modifications to the established total internal reflection fluorescence microscopy, cytoplasmic dynein molecules in the cytoplasm and on the microtubules can be visualized and their intracellular dynamics can be studied. We illustrate a technique to study motor behavior, which is not apparent in conventional ensemble studies of motors. In general, this technique can be employed to study single-molecule dynamics of fluorescently tagged proteins in the cell interior.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Thüm
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:6019
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