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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2015 (arch)     Display Documents



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ID: 717991.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2015 (arch)
Sustained Pax6 Expression Generates Primate-like Basal Radial Glia in Developing Mouse Neocortex.
Authors:Wong, Fong Kuan; Fei, Jifeng; Mora-Bermúdez, Felipe; Taverna, Elena; Haffner, Christiane; Fu, Jun; Anastassiadis, Konstantinos; Stewart, A Francis; Huttner, Wieland B.
Date of Publication (YYYY-MM-DD):2015
Title of Journal:PLoS Biology
Volume:13
Issue / Number:8
Sequence Number of Article:e1002217
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:The evolutionary expansion of the neocortex in mammals has been linked to enlargement of the subventricular zone (SVZ) and increased proliferative capacity of basal progenitors (BPs), notably basal radial glia (bRG). The transcription factor Pax6 is known to be highly expressed in primate, but not mouse, BPs. Here, we demonstrate that sustaining Pax6 expression selectively in BP-genic apical radial glia (aRG) and their BP progeny of embryonic mouse neocortex suffices to induce primate-like progenitor behaviour. Specifically, we conditionally expressed Pax6 by in utero electroporation using a novel, Tis21-CreERT2 mouse line. This expression altered aRG cleavage plane orientation to promote bRG generation, increased cell-cycle re-entry of BPs, and ultimately increased upper-layer neuron production. Upper-layer neuron production was also increased in double-transgenic mouse embryos with sustained Pax6 expression in the neurogenic lineage. Strikingly, increased BPs existed not only in the SVZ but also in the intermediate zone of the neocortex of these double-transgenic mouse embryos. In mutant mouse embryos lacking functional Pax6, the proportion of bRG among BPs was reduced. Our data identify specific Pax6 effects in BPs and imply that sustaining this Pax6 function in BPs could be a key aspect of SVZ enlargement and, consequently, the evolutionary expansion of the neocortex.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Thüm
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:6281
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