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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2015 (arch)     Display Documents



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ID: 718006.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2015 (arch)
Hyperspectral light sheet microscopy.
Authors:Jahr, Wiebke; Schmid, Benjamin; Schmied, Christopher; Fahrbach, Florian; Huisken, Jan
Date of Publication (YYYY-MM-DD):2015
Title of Journal:Nature Communications
Volume:6
Sequence Number of Article:7990
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:To study the development and interactions of cells and tissues, multiple fluorescent markers need to be imaged efficiently in a single living organism. Instead of acquiring individual colours sequentially with filters, we created a platform based on line-scanning light sheet microscopy to record the entire spectrum for each pixel in a three-dimensional volume. We evaluated data sets with varying spectral sampling and determined the optimal channel width to be around 5 nm. With the help of these data sets, we show that our setup outperforms filter-based approaches with regard to image quality and discrimination of fluorophores. By spectral unmixing we resolved overlapping fluorophores with up to nanometre resolution and removed autofluorescence in zebrafish and fruit fly embryos.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Thüm
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:6280
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