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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2015 (arch)     Display Documents



ID: 718025.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2015 (arch)
A Human Interactome in Three Quantitative Dimensions Organized by Stoichiometries and Abundances.
Authors:Hein, Marco Y; Hubner, Nina C; Poser, Ina; Cox, Jürgen; Nagaraj, Nagarjuna; Toyoda, Yusuke; Gak, Igor A; Weisswange, Ina; Mansfeld, Jorg; Buchholz, Frank; Hyman, Anthony; Mann, Matthias
Date of Publication (YYYY-MM-DD):2015
Title of Journal:Cell
Volume:163
Issue / Number:3
Start Page:712
End Page:723
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:The organization of a cell emerges from the interactions in protein networks. The interactome is critically dependent on the strengths of interactions and the cellular abundances of the connected proteins, both of which span orders of magnitude. However, these aspects have not yet been analyzed globally. Here, we have generated a library of HeLa cell lines expressing 1,125 GFP-tagged proteins under near-endogenous control, which we used as input for a next-generation interaction survey. Using quantitative proteomics, we detect specific interactions, estimate interaction stoichiometries, and measure cellular abundances of interacting proteins. These three quantitative dimensions reveal that the protein network is dominated by weak, substoichiometric interactions that play a pivotal role in defining network topology. The minority of stable complexes can be identified by their unique stoichiometry signature. This study provides a rich interaction dataset connecting thousands of proteins and introduces a framework for quantitative network analysis.
External Publication Status:published
Document Type:Article
Communicated by:Thüm
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:6374
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