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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2015 (arch)     Display Documents



ID: 718082.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2015 (arch)
Centrosomes. Regulated assembly of a supramolecular centrosome scaffold in vitro.
Authors:Woodruff, Jeffrey; Wueseke, Oliver; Viscardi, Valeria; Mahamid, J.; Ochoa, Stacy D; Bunkenborg, Jakob; Widlund, Per; Pozniakovsky, Andrei I.; Zanin, Esther; Bahmanyar, Shirin; Zinke, Andrea; Hong, Sun Hae; Decker, Markus; Baumeister, W.; Andersen, Jens S; Oegema, Karen; Hyman, Anthony
Date of Publication (YYYY-MM-DD):2015
Title of Journal:Science (New York, N.Y.)
Volume:348
Issue / Number:6236
Start Page:808
End Page:812
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:The centrosome organizes microtubule arrays within animal cells and comprises two centrioles surrounded by an amorphous protein mass called the pericentriolar material (PCM). Despite the importance of centrosomes as microtubule-organizing centers, the mechanism and regulation of PCM assembly are not well understood. In Caenorhabditis elegans, PCM assembly requires the coiled-coil protein SPD-5. We found that recombinant SPD-5 could polymerize to form micrometer-sized porous networks in vitro. Network assembly was accelerated by two conserved regulators that control PCM assembly in vivo, Polo-like kinase-1 and SPD-2/Cep192. Only the assembled SPD-5 networks, and not unassembled SPD-5 protein, functioned as a scaffold for other PCM proteins. Thus, PCM size and binding capacity emerge from the regulated polymerization of one coiled-coil protein to form a porous network.
External Publication Status:published
Document Type:Article
Communicated by:Thüm
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:6225
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