Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2015 (arch)     Display Documents



  history
ID: 718085.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2015 (arch)
Nuclear accessibility of β-actin mRNA is measured by 3D single-molecule real-time tracking.
Authors:Smith, Carlas S; Preibisch, Stephan; Joseph, Aviva; Abrahamsson, Sara; Rieger, Bernd; Myers, Eugene; Singer, Robert H.; Grunwald, David
Date of Publication (YYYY-MM-DD):2015
Title of Journal:The Journal of Cell Biology
Volume:209
Issue / Number:4
Start Page:609
End Page:619
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Imaging single proteins or RNAs allows direct visualization of the inner workings of the cell. Typically, three-dimensional (3D) images are acquired by sequentially capturing a series of 2D sections. The time required to step through the sample often impedes imaging of large numbers of rapidly moving molecules. Here we applied multifocus microscopy (MFM) to instantaneously capture 3D single-molecule real-time images in live cells, visualizing cell nuclei at 10 volumes per second. We developed image analysis techniques to analyze messenger RNA (mRNA) diffusion in the entire volume of the nucleus. Combining MFM with precise registration between fluorescently labeled mRNA, nuclear pore complexes, and chromatin, we obtained globally optimal image alignment within 80-nm precision using transformation models. We show that β-actin mRNAs freely access the entire nucleus and fewer than 60% of mRNAs are more than 0.5 µm away from a nuclear pore, and we do so for the first time accounting for spatial inhomogeneity of nuclear organization.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Thüm
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:6240
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.