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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 4 - Evolutionary Biology (R. Sommer)     Display Documents

ID: 718300.0, MPI für Entwicklungsbiologie / Abteilung 4 - Evolutionary Biology (R. Sommer)
A protocol for chemical mutagenesis in Strongyloides ratti
Authors:Guo, L.; Chang, Z.; Dieterich, C.; Streit, A.
Date of Publication (YYYY-MM-DD):2015-11
Title of Journal:Experimental Parasitology
Journal Abbrev.:Exp Parasitol
Start Page:2
End Page:7
Sequence Number of Article:25765558
Review Status:Internal review
Audience:Experts Only
Abstract / Description:Genetic analysis using experimentally induced mutations has been a most valuable tool in the analysis of various organisms. However, genetic analysis of endoparasitic organisms tends to be difficult because of the limited accessibility of the sexually reproducing adults, which are normally located within the host. Nematodes of the genera Strogyloides and Parastrongyloides represent an exception to this because they can form facultative free-living sexually reproducing generations in between parasitic generations. Here we present a protocol for the chemical mutagenesis of Strongyloides ratti. Further we evaluate the feasibility of identifying the induced mutations by whole genome re-sequencing.
Free Keywords:Animals; Ethyl Methanesulfonate/pharmacology; Feasibility Studies; Female; Genome, Helminth/drug effects/*physiology; Genome-Wide Association Study; Mutagenesis/drug effects/*physiology; Mutagens/pharmacology; Rats; Rats, Wistar; Sequence Analysis, DNA; Strongyloides ratti/drug effects/*genetics; Mutagenesis; Selection lines; Strongyloides ratti; Whole genome re-sequencing
External Publication Status:published
Document Type:Article
Communicated by:MPI für Entwickungsbiologie
Affiliations:MPI für Entwicklungsbiologie/Abteilung 4 - Evolutionsbiologie (Ralf J. Sommer)
External Affiliations:Max Delbruck Center for Molecular Medicine, Robert-Rossle-Strasse 10, 13092 Berlin, Germany. Max Planck Institute for the Biology of Ageing, Joseph-Stelzmann Strasse 9b, D-50931 Koln, Germany. Department Evolutionary Biology, Max Planck Institute for Developmental Biology, Spemannstrasse 35, D-72076 Tubingen, Germany. Electronic address: adrian.streit@tuebingen.mpg.de.
Relations:Has References-DOI:10.1016/j.exppara.2015.03.001
Identifiers:ISSN:1090-2449 (Electronic) 0014-4894 (Linking) %R 10.1... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/pubmed/25765558 [ID No:2]
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