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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 5 - Cell Biology (G. Jürgens)     Display Documents



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ID: 718760.0, MPI für Entwicklungsbiologie / Abteilung 5 - Cell Biology (G. Jürgens)
Profiling of embryonic nuclear vs. cellular RNA in Arabidopsis thaliana
Authors:Slane, D.; Kong, J.; Schmid, M.; Jurgens, G.; Bayer, M.
Language:English
Date of Publication (YYYY-MM-DD):2015-06
Title of Journal:Genom Data
Journal Abbrev.:Genom Data
Volume:4
Start Page:96
End Page:98
Sequence Number of Article:26484189
Review Status:Internal review
Audience:Experts Only
Abstract / Description:In Arabidopsis, various cell type-specific whole-genome expression analyses have been conducted. However, the vast majority of these were performed with cellular RNA from root tissues or other easily accessible cell types [1]. Nuclear RNA was neglected for a long time as not being representative for transcriptomic studies. In recent years, however, there have been reports describing the validity of nuclear RNA for these types of studies [2,3]. Here we describe the generation, quality assessment and analysis of nuclear transcriptomic data from Arabidopsis embryos published by Slane et al. (2014) [4]. Comparison of nuclear with cellular gene expression demonstrated the usefulness of nuclear transcriptomics.
Free Keywords:Arabidopsis thaliana; Gene expression; Microarray; Nuclear and cellular transcriptome; Pro-embryo and suspensor transcriptome
External Publication Status:published
Document Type:Article
Communicated by:edocabt5
Affiliations:MPI für Entwicklungsbiologie/Abteilung 5 - Zellbiologie (Gerd Jürgens)
External Affiliations:Department of Molecular Biology, Max Planck Institute for Developmental Biology, Tubingen 72076, Germany. Department of Cell Biology, Max Planck Institute for Developmental Biology, Tubingen 72076, Germany ; Department of Developmental Genetics, Center for Plant Molecular Biology, University of Tubingen, Tubingen 72076, Germany.
Identifiers:ISSN:2213-5960 (Electronic) 2213-5960 (Linking) %R 10.1... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/pubmed/26484189 [ID No:2]
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