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          Institute: MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut)     Collection: Yearbook 2016     Display Documents



ID: 723902.0, MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut) / Yearbook 2016
Semaphorin-Plexin Signaling Controls Mitotic Spindle Orientation during Epithelial Morphogenesis and Repair
Authors:Xia, J.; Swiercz, J. M.; Banon-Rodriguez, I.; Matkovic, I.; Federico, G.; Sun, T.; Franz, T.; Brakebusch, C. H.; Kumanogoh, A.; Friedel, R. H.; Martin-Belmonte, F.; Grone, H. J.; Offermanns, S.; Worzfeld, T.
Date of Publication (YYYY-MM-DD):2015-05-04
Title of Journal:Dev Cell
Volume:33
Issue / Number:3
Start Page:299
End Page:313
Audience:Not Specified
Abstract / Description:Morphogenesis, homeostasis, and regeneration of epithelial tissues rely on the accurate orientation of cell divisions, which is specified by the mitotic spindle axis. To remain in the epithelial plane, symmetrically dividing epithelial cells align their mitotic spindle axis with the plane. Here, we show that this alignment depends on epithelial cell-cell communication via semaphorin-plexin signaling. During kidney morphogenesis and repair, renal tubular epithelial cells lacking the transmembrane receptor Plexin-B2 or its semaphorin ligands fail to correctly orient the mitotic spindle, leading to severe defects in epithelial architecture and function. Analyses of a series of transgenic and knockout mice indicate that Plexin-B2 controls the cell division axis by signaling through its GTPase-activating protein (GAP) domain and Cdc42. Our data uncover semaphorin-plexin signaling as a central regulatory mechanism of mitotic spindle orientation necessary for the alignment of epithelial cell divisions with the epithelial plane.
Free Keywords:Animals; Cell Adhesion Molecules/genetics/*metabolism; Cell Communication/genetics; Cell Division/*physiology; Cell Polarity/physiology; Epithelial Cells/cytology; Epithelium/metabolism; GTPase-Activating Proteins/metabolism; Kidney/embryology/*metabolism; Male; Mice; Morphogenesis/*physiology; Nerve Tissue Proteins/genetics/*metabolism; Semaphorins/genetics/*metabolism; *Signal Transduction/physiology; Spindle Apparatus/genetics/*metabolism; Wound Healing/genetics
External Publication Status:published
Document Type:Article
Communicated by:n.n.
Affiliations:MPI für physiologische und klinische Forschung
External Affiliations:Centro de Biologia Molecular Severo Ochoa, 28049 Madrid, Spain. Institute of Pharmacology, Biochemical-Pharmacological Center (BPC), University of Marburg, 35043 Marburg, Germany. Department of Cellular and Molecular Pathology, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany. Biotech Research and Innovation Centre, University of Copenhagen, 2200 Copenhagen, Denmark. Department of Respiratory Medicine, Allergy and Rheumatic Diseases, Osaka University, Osaka 565-0871, Japan. Department of Neuroscience, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA. Department of Pharmacology, Max-Planck-Institute for Heart and Lung Research, 61231 Bad Nauheim, Germany; Medical Faculty, University of Frankfurt, 60590 Frankfurt, Germany. Department of Pharmacology, Max-Planck-Institute for Heart and Lung Research, 61231 Bad Nauheim, Germany; Institute of Pharmacology, Biochemical-Pharmacological Center (BPC), University of Marburg, 35043 Marburg, Germany. Electronic address: thomas.worzfeld@staff.uni-marburg.de.
Identifiers:ISSN:1878-1551 (Electronic) 1534-5807 (Linking) %R 10.1016/j.devcel.2015.02.001
URL:http://www.ncbi.nlm.nih.gov/pubmed/25892012
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