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          Institute: MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut)     Collection: Yearbook 2016     Display Documents



ID: 723950.0, MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut) / Yearbook 2016
Characterization of the platelet-derived growth factor receptor-alpha-positive cell lineage during murine late lung development
Authors:Ntokou, A.; Klein, F.; Dontireddy, D.; Becker, S.; Bellusci, S.; Richardson, W. D.; Szibor, M.; Braun, T.; Morty, R. E.; Seeger, W.; Voswinckel, R.; Ahlbrecht, K.
Date of Publication (YYYY-MM-DD):2015-11-01
Title of Journal:Am J Physiol Lung Cell Mol Physiol
Volume:309
Issue / Number:9
Start Page:L942
End Page:58
Audience:Not Specified
Abstract / Description:A reduced number of alveoli is the structural hallmark of diseases of the neonatal and adult lung, where alveoli either fail to develop (as in bronchopulmonary dysplasia), or are progressively destroyed (as in chronic obstructive pulmonary disease). To correct the loss of alveolar septa through therapeutic regeneration, the mechanisms of septa formation must first be understood. The present study characterized platelet-derived growth factor receptor-alpha-positive (PDGFRalpha(+)) cell populations during late lung development in mice. PDGFRalpha(+) cells (detected using a PDGFRalpha(GFP) reporter line) were noted around the proximal airways during the pseudoglandular stage. In the canalicular stage, PDGFRalpha(+) cells appeared in the more distal mesenchyme, and labeled alpha-smooth muscle actin-positive tip cells in the secondary crests and lipofibroblasts in the primary septa during alveolarization. Some PDGFRalpha(+) cells appeared in the mesenchyme of the adult lung. Over the course of late lung development, PDGFRalpha(+) cells consistently expressed collagen I, and transiently expressed markers of mesenchymal stem cells. With the use of both, a constitutive and a conditional PDGFRalpha(Cre) line, it was observed that PDGFRalpha(+) cells generated alveolar myofibroblasts including tip cells of the secondary crests, and lipofibroblasts. These lineages were committed before secondary septation. The present study provides new insights into the time-dependent commitment of the PDGFRalpha(+) cell lineage to lipofibroblasts and myofibroblasts during late lung development that is needed to better understand the cellular contribution to the process of alveolarization.
Free Keywords:fibroblast precursor; lipofibroblast; myofibroblast; septation
External Publication Status:published
Document Type:Article
Communicated by:n.n.
Affiliations:MPI für physiologische und klinische Forschung
External Affiliations:Department of Internal Medicine (Pulmonology), University of Giessen and Marburg Lung Center, Member of the German Center for Lung Research (DZL), Giessen, Germany; Wolfson Institute for Biomedical Research, University College London, London, United Kingdom; and. Department of Cardiac Development and Remodelling, Max Planck Institute for Heart and Lung Research, Member of the German Center for Lung Research (DZL), Bad Nauheim, Germany. Department of Lung Development and Remodelling, Max Planck Institute for Heart and Lung Research, Member of the German Center for Lung Research [Deutsches Zentrum fur Lungenforschung (DZL)], Bad Nauheim, Germany; Department of Internal Medicine (Pulmonology), University of Giessen and Marburg Lung Center, Member of the German Center for Lung Research (DZL), Giessen, Germany; Department of Lung Development and Remodelling, Max Planck Institute for Heart and Lung Research, Member of the German Center for Lung Research [Deutsches Zentrum fur Lungenforschung (DZL)], Bad Nauheim, Germany; Department of Internal Medicine (Pulmonology), University of Giessen and Marburg Lung Center, Member of the German Center for Lung Research (DZL), Giessen, Germany; katrin.ahlbrecht@mpi-bn.mpg.de.
Identifiers:ISSN:1522-1504 (Electronic) 1040-0605 (Linking) %R 10.1152/ajplung.00272.2014
URL:http://www.ncbi.nlm.nih.gov/pubmed/26320158
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