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          Institute: MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut)     Collection: Yearbook 2016     Display Documents



ID: 723969.0, MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut) / Yearbook 2016
Enrichment and identification of Delta(9)-Tetrahydrocannabinolic acid synthase from Pichia pastoris culture supernatants
Authors:Lange, K.; Poetsch, A.; Schmid, A.; Julsing, M. K.
Date of Publication (YYYY-MM-DD):2015-09
Title of Journal:Data Brief
Volume:4
Start Page:641
End Page:649
Audience:Not Specified
Abstract / Description:This data article refers to the report Delta(9)-Tetrahydrocannabinolic acid synthase (THCAS) production in Pichia pastoris enables chemical synthesis of cannabinoids (Lange et. al. 2015) [2]. THCAS was produced on a 2 L lab scale using recombinant P. pastoris KM71 KE1. Enrichment of THCAS as a technically pure enzyme was realized using dialysis and cationic exchange chromatography. nLC-ESI-MS/MS analysis identified THCAS in different fractions obtained by cationic exchange chromatography.
External Publication Status:published
Document Type:Article
Communicated by:n.n.
Affiliations:MPI für physiologische und klinische Forschung
External Affiliations:Plant Biochemistry, Ruhr University Bochum, 44801 Bochum, Germany. Department of Solar Materials, Helmholtz Centre for Environmental Research (UFZ), 04318 Leipzig, Germany. Laboratory of Chemical Biotechnology, Department of Biochemical & Chemical Engineering, TU Dortmund University, 44227 Dortmund, Germany.
Identifiers:ISSN:2352-3409 (Electronic) 2352-3409 (Linking) %R 10.1016/j.dib.2015.07.033
URL:http://www.ncbi.nlm.nih.gov/pubmed/26401520
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