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          Institute: MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut)     Collection: Yearbook 2016     Display Documents



ID: 723986.0, MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut) / Yearbook 2016
Dissection of metabolic pathways in the Db/Db mouse model by integrative proteome and acetylome analysis
Authors:Holper, S.; Nolte, H.; Bober, E.; Braun, T.; Kruger, M.
Date of Publication (YYYY-MM-DD):2015-03
Title of Journal:Mol Biosyst
Volume:11
Issue / Number:3
Start Page:908
End Page:922
Audience:Not Specified
Abstract / Description:Insulin resistance is often associated with excessive caloric intake and metabolic syndrome (MS) favours the development of Diabetes Mellitus Type II (T2DM). T2DM is a chronic disease with severe long-term consequences, such as dyslipidemia, retinopathy, kidney failure, and cardiovascular diseases. Although studied extensively, several aspects of T2DM remain poorly understood. Liver is the leading organ in the maintenance of metabolic fitness serving as the first relay station for processing dietary information in a direct response to nutritional input and changes in insulin and other endocrine signals. Evidence from several murine models suggests a unique function of the liver in the development of MS and T2DM. Here, we utilised Db/Db mice to understand the impact of T2DM on the proteome of liver cells. Global analysis of the liver proteome using a SILAC approach identified 407 significantly regulated proteins under diabetic conditions out of 8500 identified liver proteins. Furthermore, we mapped 1604 different acetylation sites in liver proteins. After normalization of the protein level, we identified 34 regulated acetyl lysine residues on 21 individual proteins, which were significantly altered in Db/Db compared to wild-type livers. We reason that the dataset provides a versatile resource for functional studies aiming to understand consequences of changes in protein abundances and acetylation in livers of diabetic animals.
Free Keywords:Acetylation; Adaptation, Physiological; Amino Acids/metabolism; Animals; Biological Transport; Carbohydrate Metabolism; Cluster Analysis; Diabetes Mellitus, Type 2/*metabolism; Disease Models, Animal; Fatty Acids/metabolism; Liver/metabolism; Male; *Metabolic Networks and Pathways; Mice; Microsomes/metabolism; Obesity/metabolism; Oxidation-Reduction; *Proteome; *Proteomics/methods
External Publication Status:published
Document Type:Article
Communicated by:n.n.
Affiliations:MPI für physiologische und klinische Forschung
Identifiers:ISSN:1742-2051 (Electronic) 1742-2051 (Linking) %R 10.1039/c4mb00490f
URL:http://www.ncbi.nlm.nih.gov/pubmed/25592279
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