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          Institute: MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut)     Collection: Yearbook 2016     Display Documents



  history
ID: 723994.0, MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut) / Yearbook 2016
A K(+)-selective CNG channel orchestrates Ca(2+) signalling in zebrafish sperm
Authors:Fechner, S.; Alvarez, L.; Bonigk, W.; Muller, A.; Berger, T. K.; Pascal, R.; Trotschel, C.; Poetsch, A.; Stolting, G.; Siegfried, K. R.; Kremmer, E.; Seifert, R.; Kaupp, U. B.
Date of Publication (YYYY-MM-DD):2015
Title of Journal:eLife
Volume:4
Audience:Not Specified
Abstract / Description:Calcium in the flagellum controls sperm navigation. In sperm of marine invertebrates and mammals, Ca(2+) signalling has been intensely studied, whereas for fish little is known. In sea urchin sperm, a cyclic nucleotide-gated K(+) channel (CNGK) mediates a cGMP-induced hyperpolarization that evokes Ca(2+) influx. Here, we identify in sperm of the freshwater fish Danio rerio a novel CNGK family member featuring non-canonical properties. It is located in the sperm head rather than the flagellum and is controlled by intracellular pH, but not cyclic nucleotides. Alkalization hyperpolarizes sperm and produces Ca(2+) entry. Ca(2+) induces spinning-like swimming, different from swimming of sperm from other species. The "spinning" mode probably guides sperm into the micropyle, a narrow entrance on the surface of fish eggs. A picture is emerging of sperm channel orthologues that employ different activation mechanisms and serve different functions. The channel inventories probably reflect adaptations to species-specific challenges during fertilization.
Free Keywords:biophysics; cell biology; fertilization; potassium channel; sperm signalling; structural biology; zebrafish
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:n.n.
Affiliations:MPI für physiologische und klinische Forschung
External Affiliations:Lehrstuhl Biochemie der Pflanzen, Ruhr-Universitat Bochum, Bochum, Germany. Institute of Complex Systems 4, Forschungszentrum Julich, Julich, Germany. Biology Department, University of Massachusetts Boston, Boston, United States. Institut fur Molekulare Immunologie, Helmholtz-Zentrum Munchen, Munchen, Germany.
Identifiers:ISSN:2050-084X (Electronic) 2050-084X (Linking) %R 10.7554/eLife.07624
URL:http://www.ncbi.nlm.nih.gov/pubmed/26650356
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