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          Institute: MPI für molekulare Biomedizin     Collection: Jahrbuch 2017 (publ. 2016, arch)     Display Documents



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ID: 732098.0, MPI für molekulare Biomedizin / Jahrbuch 2017 (publ. 2016, arch)
C3G/Rapgef1 Is Required in Multipolar Neurons for the Transition to a Bipolar Morphology during Cortical Development
Authors:Shah, B.; Lutter, D.; Bochenek, M. L.; Kato, K.; Tsytsyura, Y.; Glyvuk, N.; Sakakibara, A.; Klingauf, J.; Adams, R. H.; Puschel, A. W.
Date of Publication (YYYY-MM-DD):2016-04
Title of Journal:PLoS ONE
Volume:11
Issue / Number:4
Start Page:22
Review Status:Internal review
Audience:Not Specified
Abstract / Description:The establishment of a polarized morphology is essential for the development and function of neurons. During the development of the mammalian neocortex, neurons arise in the ventricular zone (VZ) from radial glia cells (RGCs) and leave the VZ to generate the cortical plate (CP). During their migration, newborn neurons first assume a multipolar morphology in the subventricular zone (SVZ) and lower intermediate zone (IZ). Subsequently, they undergo a multi-to-bipolar (MTB) transition to become bipolar in the upper IZ by developing a leading process and a trailing axon. The small GTPases Rap1A and Rap1B act as master regulators of neural cell polarity in the developing mouse neocortex. They are required for maintaining the polarity of RGCs and directing the MTB transition of multipolar neurons. Here we show that the Rap1 guanine nucleotide exchange factor (GEF) C3G (encoded by the Rapgef1 gene) is a crucial regulator of the MTB transition in vivo by conditionally inactivating the Rapgef1 gene in the developing mouse cortex at different time points during neuronal development. Inactivation of C3G results in defects in neuronal migration, axon formation and cortical lamination. Live cell imaging shows that C3G is required in cortical neurons for both the specification of an axon and the initiation of radial migration by forming a leading process.
Free Keywords:developing cerebral-cortex; nucleotide exchange factor; pyramidal; neurons; radial glia; migration; reelin; polarity; activation; rap1; neocortex; Science & Technology - Other Topics
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Jeanine Müller-Keuker
Affiliations:MPI für molekulare Biomedizin
External Affiliations:Puschel, AW (reprint author), Univ Munster, Inst Mol Zellbiol, Schlosspl 5, D-48149 Munster, Germany.; Puschel, AW (reprint author), Univ Munster, Cells In Mot Cluster Excellence, D-48149 Munster, Germany. apuschel@uni-muenster.de %G English
Identifiers:ISSN:1932-6203 %R 10.1371/journal.pone.0154174 %] e0154... [ID No:1]
URL:<Go to ISI>://WOS:000374970600041 [ID No:2]
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