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          Institute: MPI für molekulare Biomedizin     Collection: Jahrbuch 2017 (publ. 2016, arch)     Display Documents



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ID: 732119.0, MPI für molekulare Biomedizin / Jahrbuch 2017 (publ. 2016, arch)
Influence of Surface Modifications on the Spatiotemporal Microdistribution of Quantum Dots In Vivo
Authors:Nekolla, K.; Kick, K.; Sellner, S.; Mildner, K.; Zahler, S.; Zeuschner, D.; Krombach, F.; Rehberg, M.
Date of Publication (YYYY-MM-DD):2016-05
Title of Journal:Small
Volume:12
Issue / Number:19
Start Page:2641
End Page:2651
Review Status:Internal review
Audience:Not Specified
Abstract / Description:For biomedical applications of nanoconstructs, it is a general prerequisite to efficiently reach the desired target site. In this regard, it is crucial to determine the spatiotemporal distribution of nanomaterials at the microscopic tissue level. Therefore, the effect of different surface modifications on the distribution of microinjected quantum dots (QDs) in mouse skeletal muscle tissue has been investigated. In vivo real-time fluorescence microscopy and particle tracking reveal that carboxyl QDs preferentially attach to components of the extracellular matrix (ECM), whereas QDs coated with polyethylene glycol (PEG) show little interaction with tissue constituents. Transmission electron microscopy elucidates that carboxyl QDs adhere to collagen fibers as well as basement membranes, a type of ECM located on the basolateral side of blood vessel walls. Moreover, carboxyl QDs have been found in endothelial junctions as well as in caveolae of endothelial cells, enabling them to translocate into the vessel lumen. The in vivo QD distribution is confirmed by in vitro experiments. The data suggest that ECM components act as a selective barrier depending on QD surface modification. For future biomedical applications, such as targeting of blood vessel walls, the findings of this study offer design criteria for nanoconstructs that meet the requirements of the respective application.
Free Keywords:endothelial cells; extracellular matrix; in vivo microscopy; nanoparticles; transcytosis
External Publication Status:published
Document Type:Article
Communicated by:Jeanine Müller-Keuker
Affiliations:MPI für molekulare Biomedizin
External Affiliations:Department of Pharmacy, Ludwig-Maximilians-Universitat Munchen, Butenandtstr. 5-13, 81377, Munich, Germany. Max Planck Institute for Molecular Biomedicine, Rontgenstr. 20, 48149, Munster, Germany.
Identifiers:ISSN:1613-6829 (Electronic) 1613-6810 (Linking) %R 10.1... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/pubmed/27028603 [ID No:2]
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