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          Institute: MPI für molekulare Biomedizin     Collection: Jahrbuch 2017 (publ. 2016, arch)     Display Documents



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ID: 732129.0, MPI für molekulare Biomedizin / Jahrbuch 2017 (publ. 2016, arch)
Establishment of feeder-free culture system for human induced pluripotent stem cell on DAS nanocrystalline graphene
Authors:Lee, H.; Nam, D.; Choi, J. K.; Arauzo-Bravo, M. J.; Kwon, S. Y.; Zaehres, H.; Lee, T.; Park, C. Y.; Kang, H. W.; Scholer, H. R.; Kim, J. B.
Date of Publication (YYYY-MM-DD):2016-02-05
Title of Journal:Sci Rep
Volume:6
Start Page:20708
Review Status:Internal review
Audience:Not Specified
Abstract / Description:The maintenance of undifferentiated human pluripotent stem cells (hPSC) under xeno-free condition requires the use of human feeder cells or extracellular matrix (ECM) coating. However, human-derived sources may cause human pathogen contamination by viral or non-viral agents to the patients. Here we demonstrate feeder-free and xeno-free culture system for hPSC expansion using diffusion assisted synthesis-grown nanocrystalline graphene (DAS-NG), a synthetic non-biological nanomaterial which completely rule out the concern of human pathogen contamination. DAS-NG exhibited advanced biocompatibilities including surface nanoroughness, oxygen containing functional groups and hydrophilicity. hPSC cultured on DAS-NG could maintain pluripotency in vitro and in vivo, and especially cell adhesion-related gene expression profile was comparable to those of cultured on feeders, while hPSC cultured without DAS-NG differentiated spontaneously with high expression of somatic cell-enriched adhesion genes. This feeder-free and xeno-free culture method using DAS-NG will facilitate the generation of clinical-grade hPSC.
Free Keywords:Cell Adhesion; Cell Culture Techniques/*methods; Cell Differentiation; Cell Line; Cell Proliferation; Feeder Cells/cytology; Gene Expression; Graphite/*chemistry; Humans; Induced Pluripotent Stem Cells/*cytology/metabolism; Nanostructures/*chemistry; Surface Properties
External Publication Status:published
Document Type:Article
Communicated by:Jeanine Müller-Keuker
Affiliations:MPI für molekulare Biomedizin
External Affiliations:SMEs Support Center, Korea Institute of Science and Technology Information, 48058 Busan, South Korea. Group of Computational Biology and Bioinformatics, Biodonostia Health Research Institute, 20014 San Sebastian, Spain. IKERBASQUE, Basque Foundation for Science, Bilbao, Spain. School of Materials Science and Engineering, Ulsan National Institute of Science and Technology (UNIST), 44919 Ulsan, South Korea. Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Rontgenstrasse 20, 48149 Munster, Germany.
Identifiers:ISSN:2045-2322 (Electronic) 2045-2322 (Linking) %R 10.1... [ID No:1]
URL:https://www.ncbi.nlm.nih.gov/pubmed/26846167 [ID No:2]
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