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          Institute: MPI für molekulare Biomedizin     Collection: Jahrbuch 2017 (publ. 2016, arch)     Display Documents

ID: 732132.0, MPI für molekulare Biomedizin / Jahrbuch 2017 (publ. 2016, arch)
In vivo modulation of endothelial polarization by Apelin receptor signalling
Authors:Kwon, H. B.; Wang, S.; Helker, C. S.; Rasouli, S. J.; Maischein, H. M.; Offermanns, S.; Herzog, W.; Stainier, D. Y.
Date of Publication (YYYY-MM-DD):2016-06-01
Title of Journal:Nat Commun
Start Page:11805
Review Status:Internal review
Audience:Not Specified
Abstract / Description:Endothelial cells (ECs) respond to shear stress by aligning in the direction of flow. However, how ECs respond to flow in complex in vivo environments is less clear. Here we describe an endothelial-specific transgenic zebrafish line, whereby the Golgi apparatus is labelled to allow for in vivo analysis of endothelial polarization. We find that most ECs polarize within 4.5 h after the onset of vigorous blood flow and, by manipulating cardiac function, observe that flow-induced EC polarization is a dynamic and reversible process. Based on its role in EC migration, we analyse the role of Apelin signalling in EC polarization and find that it is critical for this process. Knocking down Apelin receptor function in human primary ECs also affects their polarization. Our study provides new tools to analyse the mechanisms of EC polarization in vivo and reveals an important role in this process for a signalling pathway implicated in cardiovascular disease.
External Publication Status:published
Document Type:Article
Communicated by:Jeanine Müller-Keuker
Affiliations:MPI für molekulare Biomedizin
External Affiliations:Department of Pharmacology, Max Planck Institute for Heart and Lung Research, Ludwigstrasse 43, 61231 Bad Nauheim, Germany. University of Munster, Schlossplatz 2, 48149 Munster, Germany. Max Planck Institute for Molecular Biomedicine, Rontgenstrasse 20, 48149 Munster, Germany.
Identifiers:ISSN:2041-1723 (Electronic) 2041-1723 (Linking) %R 10.1... [ID No:1]
URL:https://www.ncbi.nlm.nih.gov/pubmed/27248505 [ID No:2]
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