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          Institute: MPI für molekulare Biomedizin     Collection: Jahrbuch 2017 (publ. 2016, arch)     Display Documents



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ID: 732136.0, MPI für molekulare Biomedizin / Jahrbuch 2017 (publ. 2016, arch)
Induced neural stem cells from distinct genetic backgrounds exhibit different reprogramming status
Authors:Kim, S. M.; Lim, K. T.; Kwak, T. H.; Lee, S. C.; Im, J. H.; Hali, S.; In Hwang, S.; Kim, D.; Hwang, J.; Kim, K. P.; Chung, H. J.; Kim, J. B.; Ko, K.; Chung, H. M.; Lee, H. T.; Scholer, H. R.; Han, D. W.
Date of Publication (YYYY-MM-DD):2016-03
Title of Journal:Stem Cell Res
Volume:16
Issue / Number:2
Start Page:460
End Page:468
Review Status:Internal review
Audience:Not Specified
Abstract / Description:Somatic cells could be directly converted into induced neural stem cells (iNSCs) by ectopic expression of defined transcription factors. However, the underlying mechanism of direct lineage transition into iNSCs is largely unknown. In this study, we examined the effect of genetic background on the direct conversion process into an iNSC state. The iNSCs from two different mouse strains exhibited the distinct efficiency of lineage conversion as well as clonal expansion. Furthermore, the expression levels of endogenous NSC markers, silencing of transgenes, and in vitro differentiation potential were also different between iNSC lines from different strains. Therefore, our data suggest that the genetic background of starting cells influences the conversion efficiency as well as reprogramming status of directly converted iNSCs.
Free Keywords:Animals; Cell Differentiation; Cells, Cultured; Cellular Reprogramming; Collagen Type I/metabolism; Fibroblasts/cytology; Induced Pluripotent Stem Cells/*cytology/metabolism; Male; Mice; Mice, Inbred C57BL; Microscopy, Fluorescence; Nestin/metabolism; Neural Stem Cells/*cytology/metabolism; Real-Time Polymerase Chain Reaction; Transcription Factors/genetics/metabolism; Cell fate transition; Direct conversion; Direct reprogramming; Genetic backgrounds; iNSCs
External Publication Status:published
Document Type:Article
Communicated by:Jeanine Müller-Keuker
Affiliations:MPI für molekulare Biomedizin
External Affiliations:Department of Stem Cell Biology, School of Medicine, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea. Dong-A Socio Holdings Research Center, 21, Geumhwa-ro 105 beon-gil, Giheung-gu, Yongin-si, Republic of Korea. Department of Animal Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea. Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Rontgenstrasse 20, 48149 Munster, Germany. Animal Biotechnology Division, National Institute of Animal Science, RDA, Suwon 441-706, Republic of Korea. School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan, Republic of Korea. Department of Stem Cell Biology, School of Medicine, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea; KU Open-Innovation Center, Institute of Biomedical Science & Technology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea. Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Rontgenstrasse 20, 48149 Munster, Germany; University of Munster, Medical Faculty, Domagkstrasse 3, 48149 Munster, Germany. Department of Stem Cell Biology, School of Medicine, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea; KU Open-Innovation Center, Institute of Biomedical Science & Technology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea. Electronic address: dwhan@konkuk.ac.kr.
Identifiers:ISSN:1876-7753 (Electronic) 1873-5061 (Linking) %R 10.1... [ID No:1]
URL:https://www.ncbi.nlm.nih.gov/pubmed/26930613 [ID No:2]
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