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          Institute: MPI für molekulare Biomedizin     Collection: Jahrbuch 2017 (publ. 2016, arch)     Display Documents



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ID: 732137.0, MPI für molekulare Biomedizin / Jahrbuch 2017 (publ. 2016, arch)
Generation of Integration-free Induced Neural Stem Cells from Mouse Fibroblasts
Authors:Kim, S. M.; Kim, J. W.; Kwak, T. H.; Park, S. W.; Kim, K. P.; Park, H.; Lim, K. T.; Kang, K.; Kim, J.; Yang, J. H.; Han, H.; Lee, I.; Hyun, J. K.; Bae, Y. M.; Scholer, H. R.; Lee, H. T.; Han, D. W.
Date of Publication (YYYY-MM-DD):2016-07-01
Title of Journal:J Biol Chem
Volume:291
Issue / Number:27
Start Page:14199
End Page:14212
Review Status:Internal review
Audience:Not Specified
Abstract / Description:The viral vector-mediated overexpression of the defined transcription factors, Brn4/Pou3f4, Sox2, Klf4, and c-Myc (BSKM), could induce the direct conversion of somatic fibroblasts into induced neural stem cells (iNSCs). However, viral vectors may be randomly integrated into the host genome thereby increasing the risk for undesired genotoxicity, mutagenesis, and tumor formation. Here we describe the generation of integration-free iNSCs from mouse fibroblasts by non-viral episomal vectors containing BSKM. The episomal vector-derived iNSCs (e-iNSCs) closely resemble control NSCs, and iNSCs generated by retrovirus (r-iNSCs) in morphology, gene expression profile, epigenetic status, and self-renewal capacity. The e-iNSCs are functionally mature, as they could differentiate into all the neuronal cell types both in vitro and in vivo Our study provides a novel concept for generating functional iNSCs using a non-viral, non-integrating, plasmid-based system that could facilitate their biomedical applicability.
Free Keywords:Animals; Fibroblasts/cytology; Genetic Vectors; Mice; Mice, Inbred C3H; Neural Stem Cells/*cytology; Transfection; cell biology; cell therapy; neural stem cell (NSC); regenerative medicine; reprogramming
External Publication Status:published
Document Type:Article
Communicated by:Jeanine Müller-Keuker
Affiliations:MPI für molekulare Biomedizin
External Affiliations:the Department of Nanobiomedical Science, Dankook University Graduate School, Cheonan 31116, Republic of Korea. From the Departments of Stem Cell Biology, School of Medicine, and. the Department of Physiology, School of Medicine, Konkuk University, Chungju, Chungbuk 27478, Republic of Korea. the Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Rontgenstrasse 20, 48149 Munster, Germany. the Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 04056, Korea. the Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Rontgenstrasse 20, 48149 Munster, Germany, the University of Munster, Medical Faculty, Domagkstrasse 3, 48149 Munster, Germany, and. Animal Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of Korea. From the Departments of Stem Cell Biology, School of Medicine, and the KU Open-Innovation Center, Institute of Biomedical Science & Technology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of Korea dwhan@konkuk.ac.kr.
Identifiers:ISSN:1083-351X (Electronic) 0021-9258 (Linking) %R 10.1... [ID No:1]
URL:https://www.ncbi.nlm.nih.gov/pubmed/27189941 [ID No:2]
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