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          Institute: MPI für molekulare Biomedizin     Collection: Jahrbuch 2017 (publ. 2016, arch)     Display Documents



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ID: 732141.0, MPI für molekulare Biomedizin / Jahrbuch 2017 (publ. 2016, arch)
Distinct Signaling Requirements for the Establishment of ESC Pluripotency in Late-Stage EpiSCs
Authors:Illich, D. J.; Zhang, M.; Ursu, A.; Osorno, R.; Kim, K. P.; Yoon, J.; Arauzo-Bravo, M. J.; Wu, G.; Esch, D.; Sabour, D.; Colby, D.; Grassme, K. S.; Chen, J.; Greber, B.; Hoing, S.; Herzog, W.; Ziegler, S.; Chambers, I.; Gao, S.; Waldmann, H.; Scholer, H. R.
Date of Publication (YYYY-MM-DD):2016-04-14
Title of Journal:Cell Rep
Volume:15
Issue / Number:4
Start Page:787
End Page:800
Review Status:Internal review
Audience:Not Specified
Abstract / Description:It has previously been reported that mouse epiblast stem cell (EpiSC) lines comprise heterogeneous cell populations that are functionally equivalent to cells of either early- or late-stage postimplantation development. So far, the establishment of the embryonic stem cell (ESC) pluripotency gene regulatory network through the widely known chemical inhibition of MEK and GSK3beta has been impractical in late-stage EpiSCs. Here, we show that chemical inhibition of casein kinase 1alpha (CK1alpha) induces the conversion of recalcitrant late-stage EpiSCs into ESC pluripotency. CK1alpha inhibition directly results in the simultaneous activation of the WNT signaling pathway, together with inhibition of the TGFbeta/SMAD2 signaling pathway, mediating the rewiring of the gene regulatory network in favor of an ESC-like state. Our findings uncover a molecular mechanism that links CK1alpha to ESC pluripotency through the direct modulation of WNT and TGFbeta signaling.
External Publication Status:published
Document Type:Article
Communicated by:Jeanine Müller-Keuker
Affiliations:MPI für molekulare Biomedizin
External Affiliations:Max Planck Institute for Molecular Biomedicine, Rontgenstrasse 20, 48149 Munster, Germany. Max Planck Institute for Molecular Physiology, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany; Technische Universitat Dortmund, 44227 Dortmund, Germany. Max Planck Institute for Molecular Biomedicine, Rontgenstrasse 20, 48149 Munster, Germany; IKERBASQUE, Basque Foundation for Science, 48013 Bilbao, Spain. MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh EH16 4UU, Scotland. University of Munster, 48149 Munster, Germany. School of Life Sciences and Technology, Tongji University, Shanghai 200092, China. Human Stem Cell Pluripotency Laboratory, Max Planck Institute for Molecular Biomedicine, 48149 Munster, Germany; Chemical Genomics Centre of the Max Planck Society, 44227 Dortmund, Germany. Max Planck Institute for Molecular Biomedicine, Rontgenstrasse 20, 48149 Munster, Germany; University of Munster, 48149 Munster, Germany; Cells-in-Motion Cluster of Excellence (EXC 1003 - CiM), University of Munster, 48149 Munster, Germany. Max Planck Institute for Molecular Physiology, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany. Max Planck Institute for Molecular Physiology, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany; Technische Universitat Dortmund, 44227 Dortmund, Germany. Electronic address: herbert.waldmann@mpi-dortmund.mpg.de. Max Planck Institute for Molecular Biomedicine, Rontgenstrasse 20, 48149 Munster, Germany; University of Munster, 48149 Munster, Germany. Electronic address: office@mpi-muenster.mpg.de.
Identifiers:ISSN:2211-1247 (Electronic) %R 10.1016/j.celrep.2016.03... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/pubmed/27149845 [ID No:2]
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