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          Institute: MPI für molekulare Biomedizin     Collection: Jahrbuch 2017 (publ. 2016, arch)     Display Documents



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ID: 732146.0, MPI für molekulare Biomedizin / Jahrbuch 2017 (publ. 2016, arch)
Comparative transcriptome analysis in induced neural stem cells reveals defined neural cell identities in vitro and after transplantation into the adult rodent brain
Authors:Hallmann, A. L.; Arauzo-Bravo, M. J.; Zerfass, C.; Senner, V.; Ehrlich, M.; Psathaki, O. E.; Han, D. W.; Tapia, N.; Zaehres, H.; Scholer, H. R.; Kuhlmann, T.; Hargus, G.
Date of Publication (YYYY-MM-DD):2016-05
Title of Journal:Stem Cell Res
Volume:16
Issue / Number:3
Start Page:776
End Page:781
Review Status:Internal review
Audience:Not Specified
Abstract / Description:Reprogramming technology enables the production of neural progenitor cells (NPCs) from somatic cells by direct transdifferentiation. However, little is known on how neural programs in these induced neural stem cells (iNSCs) differ from those of alternative stem cell populations in vitro and in vivo. Here, we performed transcriptome analyses on murine iNSCs in comparison to brain-derived neural stem cells (NSCs) and pluripotent stem cell-derived NPCs, which revealed distinct global, neural, metabolic and cell cycle-associated marks in these populations. iNSCs carried a hindbrain/posterior cell identity, which could be shifted towards caudal, partially to rostral but not towards ventral fates in vitro. iNSCs survived after transplantation into the rodent brain and exhibited in vivo-characteristics, neural and metabolic programs similar to transplanted NSCs. However, iNSCs vastly retained caudal identities demonstrating cell-autonomy of regional programs in vivo. These data could have significant implications for a variety of in vitro- and in vivo-applications using iNSCs.
Free Keywords:Induced neural stem cells; Neural stem cells; Regional programs; Transcriptome; Transplantation
External Publication Status:published
Document Type:Article
Communicated by:Jeanine Müller-Keuker
Affiliations:MPI für molekulare Biomedizin
External Affiliations:Group of Computational Biology and Systems Biomedicine, Biodonostia Health Research Institute, 20014 San Sebastian, Spain; IKERBASQUE, Basque Foundation for Science, 48011 Bilbao, Spain. Institute of Neuropathology, University Hospital Munster, 48149 Munster, Germany. Max Planck Institute for Molecular Biomedicine, 48149 Munster, Germany. Department of Stem Cell Biology, School of Medicine, Konkuk University, 143701 Seoul, Republic of Korea. Max Planck Institute for Molecular Biomedicine, 48149 Munster, Germany; Institute of Biomedicine of Valencia, Spanish National Research Council (IBV-CSIC), Jaime Roig 11, 46010 Valencia, Spain. Institute of Neuropathology, University Hospital Munster, 48149 Munster, Germany; Max Planck Institute for Molecular Biomedicine, 48149 Munster, Germany; Department of Pathology and Cell Biology, Columbia University Medical Center, 10032 New York, USA. Electronic address: gunnar.hargus@ukmuenster.de.
Identifiers:ISSN:1876-7753 (Electronic) 1873-5061 (Linking) %R 10.1... [ID No:1]
URL:https://www.ncbi.nlm.nih.gov/pubmed/27153350 [ID No:2]
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