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          Institute: MPI für molekulare Biomedizin     Collection: Jahrbuch 2017 (publ. 2016, arch)     Display Documents



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ID: 732162.0, MPI für molekulare Biomedizin / Jahrbuch 2017 (publ. 2016, arch)
Distinct Enhancer Activity of Oct4 in Naive and Primed Mouse Pluripotency
Authors:Choi, Hyun Woo; Joo, Jin Young; Hong, Yean Ju; Kim, Jong Soo; Song, Hyuk; Lee, Jeong Woong; Wu, Guangming; Schöler, Hans R; Do, Jeong Tae
Date of Publication (YYYY-MM-DD):2016
Title of Journal:Stem Cell Reports
Review Status:Internal review
Audience:Not Specified
Abstract / Description:Naive and primed pluripotent stem cells (PSCs) and germ cells express the Oct4 gene. The Oct4 gene contains two cis-regulatory elements, the distal enhancer (DE) and proximal enhancer (PE), which differentially control Oct4 expression in a cell-type-specific and stage-specific manner. Here, we generated double transgenic mice carrying both Oct4-?PE-GFP and Oct4-?DE-tdTomato (RFP), enabling us to simultaneously monitor the activity of DE and PE. Oct4 expression is stage-specifically regulated by DE and PE during embryonic and germ cell development. Using this dual reporter system, we successfully cultured pure populations of naive (GFP+RFP?) and primed (GFP?RFP+) PSCs. We found that GFP+RFP? cells were metastable (not naive) in serum-containing medium; stable naive pluripotent cells were observed in medium containing two inhibitors (Meki and GSKi) but lacked serum. Finally, we suggest that the activity of Oct4 DE and PE is regulated by the repressive histone marks and DNA methylation in a cell-type-specific manner.
External Publication Status:published
Document Type:Article
Communicated by:Jeanine Müller-Keuker
Affiliations:MPI für molekulare Biomedizin
Identifiers:ISSN:2213-6711 %R 10.1016/j.stemcr.2016.09.012 [ID No:1]
URL:http://dx.doi.org/10.1016/j.stemcr.2016.09.012 htt... [ID No:2]
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