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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2016 (archival)     Display Documents



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ID: 732375.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2016 (archival)
Light-sheet microscopy for everyone? Experience of building an OpenSPIM to study flatworm development.
Authors:Girstmair, Johannes; Zakrzewski, Anne; Lapraz, François; Handberg-Thorsager, Mette; Tomancak, Pavel; Pitrone, Peter Gabriel; Simpson, Fraser; Telford, Maximilian J
Date of Publication (YYYY-MM-DD):2016
Title of Journal:BMC Developmental Biology
Volume:16
Issue / Number:1
Sequence Number of Article:22
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Selective plane illumination microscopy (SPIM a type of light-sheet microscopy) involves focusing a thin sheet of laser light through a specimen at right angles to the objective lens. As only the thin section of the specimen at the focal plane of the lens is illuminated, out of focus light is naturally absent and toxicity due to light (phototoxicity) is greatly reduced enabling longer term live imaging. OpenSPIM is an open access platform (Pitrone et al. 2013 and OpenSPIM.org) created to give new users step-by-step instructions on building a basic configuration of a SPIM microscope, which can in principle be adapted and upgraded to each laboratory's own requirements and budget. Here we describe our own experience with the process of designing, building, configuring and using an OpenSPIM for our research into the early development of the polyclad flatworm Maritigrella crozieri - a non-model animal.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Thüm
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:6588
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