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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2016 (archival)     Display Documents



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ID: 732376.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2016 (archival)
Lipid Discovery by Combinatorial Screening and Untargeted LC-MS/MS.
Authors:Bilgin, Mesut; Born, Petra; Fezza, Filomena; Heimes, Michael; Mastrangelo, Nicolina; Wagner, Nicolai; Schultz, Carsten; Maccarrone, Mauro; Eaton, Suzanne; Nadler, André; Wilm, Matthias; Shevchenko, Andrej
Date of Publication (YYYY-MM-DD):2016
Title of Journal:Scientific Reports
Volume:6
Sequence Number of Article:27920
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:We present a method for the systematic identification of picogram quantities of new lipids in total extracts of tissues and fluids. It relies on the modularity of lipid structures and applies all-ions fragmentation LC-MS/MS and Arcadiate software to recognize individual modules originating from the same lipid precursor of known or assumed structure. In this way it alleviates the need to recognize and fragment very low abundant precursors of novel molecules in complex lipid extracts. In a single analysis of rat kidney extract the method identified 58 known and discovered 74 novel endogenous endocannabinoids and endocannabinoid-related molecules, including a novel class of N-acylaspartates that inhibit Hedgehog signaling while having no impact on endocannabinoid receptors.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Thüm
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:6568
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