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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2016 (archival)     Display Documents



ID: 732393.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2016 (archival)
Visualizing the molecular sociology at the HeLa cell nuclear periphery.
Authors:Mahamid, J.; Pfeffer, Stefan; Schaffer, Miroslava; Villa, Elizabeth; Danev, Radostin; Cuellar, Luis Kuhn; Förster, Friedrich; Hyman, Anthony; Plitzko, Jürgen M; Baumeister, W.
Date of Publication (YYYY-MM-DD):2016
Title of Journal:Science (New York, N.Y.)
Volume:351
Issue / Number:6276
Start Page:969
End Page:972
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:The molecular organization of eukaryotic nuclear volumes remains largely unexplored. Here we combined recent developments in cryo-electron tomography (cryo-ET) to produce three-dimensional snapshots of the HeLa cell nuclear periphery. Subtomogram averaging and classification of ribosomes revealed the native structure and organization of the cytoplasmic translation machinery. Analysis of a large dynamic structure-the nuclear pore complex-revealed variations detectable at the level of individual complexes. Cryo-ET was used to visualize previously elusive structures, such as nucleosome chains and the filaments of the nuclear lamina, in situ. Elucidation of the lamina structure provides insight into its contribution to metazoan nuclear stiffness.
External Publication Status:published
Document Type:Article
Communicated by:Thüm
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:6456
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