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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2016 (archival)     Display Documents



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ID: 732395.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2016 (archival)
Augmin shapes the anaphase spindle for efficient cytokinetic furrow ingression and abscission.
Authors:Uehara, Ryota; Kamasaki, Tomoko; Hiruma, Shota; Poser, Ina; Yoda, Kinya; Yajima, Junichiro; Gerlich, Daniel W; Goshima, Gohta
Date of Publication (YYYY-MM-DD):2016
Title of Journal:Molecular Biology of the Cell
Volume:27
Issue / Number:5
Start Page:812
End Page:827
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:During anaphase, distinct populations of microtubules (MTs) form by either centrosome-dependent or augmin-dependent nucleation. It remains largely unknown whether these different MT populations contribute distinct functions to cytokinesis. Here we show that augmin-dependent MTs are required for the progression of both furrow ingression and abscission. Augmin depletion reduced the accumulation of anillin, a contractile ring regulator at the cell equator, yet centrosomal MTs were sufficient to mediate RhoA activation at the furrow. This defect in contractile ring organization, combined with incomplete spindle pole separation during anaphase, led to impaired furrow ingression. During the late stages of cytokinesis, astral MTs formed bundles in the intercellular bridge, but these failed to assemble a focused midbody structure and did not establish tight linkage to the plasma membrane, resulting in furrow regression. Thus augmin-dependent acentrosomal MTs and centrosomal MTs contribute to nonredundant targeting mechanisms of different cytokinesis factors, which are required for the formation of a functional contractile ring and midbody.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Thüm
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:6492
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