| ID:
732462.0,
MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2016 (archival) |
| Sample Preparation and Mounting of Drosophila Embryos for Multiview Light Sheet Microscopy. |
| Authors: | Schmied, Christopher; Tomancak, Pavel |
| Date of Publication (YYYY-MM-DD): | 2016 |
| Title of Journal: | Methods in Molecular Biology (Clifton, N.J.) |
| Volume: | 1478 |
| Start Page: | 189 |
| End Page: | 202 |
| Copyright: | not available |
| Audience: | Experts Only |
| Intended Educational Use: | No |
| Abstract / Description: | Light sheet fluorescent microscopy (LSFM), and in particular its most widespread flavor Selective Plane Illumination Microscopy (SPIM), promises to provide unprecedented insights into developmental dynamics of entire living systems. By combining minimal photo-damage with high imaging speed and sample mounting tailored toward the needs of the specimen, it enables in toto imaging of embryogenesis with high spatial and temporal resolution. Drosophila embryos are particularly well suited for SPIM imaging because the volume of the embryo does not change from the single cell embryo to the hatching larva. SPIM microscopes can therefore image Drosophila embryos embedded in rigid media, such as agarose, from multiple angles every few minutes from the blastoderm stage until hatching. Here, we describe sample mounting strategies to achieve such a recording. We also provide detailed protocols to realize multiview, long-term, time-lapse recording of Drosophila embryos expressing fluorescent markers on the commercially available Zeiss Lightsheet Z.1 microscope and the OpenSPIM. |
| External Publication Status: | published |
| Document Type: | Article |
| Version Comment: | Automatic journal name synchronization |
| Communicated by: | Thüm |
| Affiliations: | MPI für molekulare Zellbiologie und Genetik
|
| Identifiers: | LOCALID:6658 |
|
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