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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2016 (archival)     Display Documents



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ID: 732466.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2016 (archival)
TALE-directed local modulation of H3K9 methylation shapes exon recognition.
Authors:Bieberstein, Nicole; Kozáková, Eva; Huranová, Martina; Thakur, Prasoon K; Krchňáková, Zuzana; Krausová, Michaela; Oesterreich, Fernando Carrillo; Staněk, David
Date of Publication (YYYY-MM-DD):2016
Title of Journal:Scientific Reports
Volume:6
Sequence Number of Article:29961
Copyright:not available
Review Status:Internal review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:In search for the function of local chromatin environment on pre-mRNA processing we established a new tool, which allows for the modification of chromatin using a targeted approach. Using Transcription Activator-Like Effector domains fused to histone modifying enzymes (TALE-HME), we show locally restricted alteration of histone methylation modulates the splicing of target exons. We provide evidence that a local increase in H3K9 di- and trimethylation promotes inclusion of the target alternative exon, while demethylation by JMJD2D leads to exon skipping. We further demonstrate that H3K9me3 is localized on internal exons genome-wide suggesting a general role in splicing. Consistently, targeting of the H3K9 demethylase to a weak constitutive exon reduced co-transcriptional splicing. Together our data show H3K9 methylation within the gene body is a factor influencing recognition of both constitutive and alternative exons.
External Publication Status:published
Document Type:Article
Communicated by:Thüm
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:6612
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