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Home Search Quick Search Advanced Fulltext Browse Collections Persons My eDoc Session History Login Name: Password: Documentation Help Support Wiki Direct access to document ID:	Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2016 (archival)     Display Documents ID: 732476.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2016 (archival) Polar Positioning of Phase-Separated Liquid Compartments in Cells Regulated by an mRNA Competition Mechanism. Authors: Saha, Shambaditya; Weber, Christoph A.; Nousch, Marco; Adame-Arana, Omar; Hoege, Carsten; Hein, Marco Y; Osborne-Nishimura, Erin; Mahamid, J.; Jahnel, Marcus; Jawerth, Louise; Pozniakovski, Andrei; Eckmann, Christian R.; Jülicher, Frank; Hyman, Anthony Date of Publication (YYYY-MM-DD): 2016 Title of Journal: Cell Volume: 166 Issue / Number: 6 Start Page: 1572 End Page: 1584 Copyright: not available Audience: Experts Only Intended Educational Use: No Abstract / Description: P granules are non-membrane-bound RNA-protein compartments that are involved in germline development in C. elegans. They are liquids that condense at one end of the embryo by localized phase separation, driven by gradients of polarity proteins such as the mRNA-binding protein MEX-5. To probe how polarity proteins regulate phase separation, we combined biochemistry and theoretical modeling. We reconstitute P granule-like droplets in vitro using a single protein PGL-3. By combining in vitro reconstitution with measurements of intracellular concentrations, we show that competition between PGL-3 and MEX-5 for mRNA can regulate the formation of PGL-3 droplets. Using theory, we show that, in a MEX-5 gradient, this mRNA competition mechanism can drive a gradient of P granule assembly with similar spatial and temporal characteristics to P granule assembly in vivo. We conclude that gradients of polarity proteins can position RNP granules during development by using RNA competition to regulate local phase separation. External Publication Status: published Document Type: Article Communicated by: Thüm Affiliations: MPI für molekulare Zellbiologie und Genetik Identifiers: LOCALID:6638 The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.