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Home Search Quick Search Advanced Fulltext Browse Collections Persons My eDoc Session History Login Name: Password: Documentation Help Support Wiki Direct access to document ID:	Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2016 (archival)     Display Documents ID: 732499.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2016 (archival) Nucleosomal arrangement affects single-molecule transcription dynamics. Authors: Fitz, Veronika; Shin, Jaeoh; Ehrlich, Christoph; Farnung, Lucas; Cramer, Patrick; Zaburdaev, Vasily; Grill, Stephan W. Date of Publication (YYYY-MM-DD): 2016 Title of Journal: Proceedings of the National Academy of Sciences of the United States of America Volume: 113 Issue / Number: 45 Start Page: 12733 End Page: 12738 Copyright: not available Audience: Experts Only Intended Educational Use: No Abstract / Description: In eukaryotes, gene expression depends on chromatin organization. However, how chromatin affects the transcription dynamics of individual RNA polymerases has remained elusive. Here, we use dual trap optical tweezers to study single yeast RNA polymerase II (Pol II) molecules transcribing along a DNA template with two nucleosomes. The slowdown and the changes in pausing behavior within the nucleosomal region allow us to determine a drift coefficient, χ, which characterizes the ability of the enzyme to recover from a nucleosomal backtrack. Notably, χ can be used to predict the probability to pass the first nucleosome. Importantly, the presence of a second nucleosome changes χ in a manner that depends on the spacing between the two nucleosomes, as well as on their rotational arrangement on the helical DNA molecule. Our results indicate that the ability of Pol II to pass the first nucleosome is increased when the next nucleosome is turned away from the first one to face the opposite side of the DNA template. These findings help to rationalize how chromatin arrangement affects Pol II transcription dynamics. External Publication Status: published Document Type: Article Communicated by: Thüm Affiliations: MPI für molekulare Zellbiologie und Genetik Identifiers: LOCALID:6690 The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.