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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: MPI-CBG Publications 2016 (archival)     Display Documents



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ID: 732525.0, MPI für molekulare Zellbiologie und Genetik / MPI-CBG Publications 2016 (archival)
Optotaxis: Caged Lysophosphatidic Acid Enables Optical Control of a Chemotactic Gradient.
Authors:Hövelmann, Felix; Kedziora, Katarzyna M; Nadler, André; Müller, Rainer; Jalink, Kees; Schultz, Carsten
Date of Publication (YYYY-MM-DD):2016
Title of Journal:Cell Chemical Biology
Volume:23
Issue / Number:5
Start Page:629
End Page:634
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Lysophosphatidic acid (LPA) is a serum-borne lipid mediator that binds to a variety of different G protein-coupled receptors to trigger an exceptionally wide range of biological effects, including cell survival and differentiation, cancer cell migration, and embryonic development. Here we synthesized caged LPA (cgLPA), a "photolysable" coumarin-masked derivative of LPA. We demonstrate that illumination of cgLPA with 405 nm light liberates bioactive LPA on a subsecond scale to evoke Ca(2+) signaling, Rho activation, and cytoskeletal contraction. In addition, we developed an "optotaxis" assay to attract melanoma cells through a stable chemotactic gradient by repeated liberation of LPA through local photolysis of extracellular cgLPA. We expect that this method of light-controlled chemotaxis will be generally applicable to a large variety of small molecules that drive cellular migration or other responses.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Thüm
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:6535
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