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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 2 - Biochemistry (E. Izaurralde)     Display Documents



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ID: 732996.0, MPI für Entwicklungsbiologie / Abteilung 2 - Biochemistry (E. Izaurralde)
miRISC and the CCR4-NOT complex silence mRNA targets independently of 43S ribosomal scanning
Authors:Kuzuoglu-Ozturk, D.; Bhandari, D.; Huntzinger, E.; Fauser, M.; Helms, S.; Izaurralde, E.
Date of Publication (YYYY-MM-DD):2016-06-01
Title of Journal:EMBO J
Volume:35
Issue / Number:11
Start Page:1186
End Page:1203
Review Status:Internal review
Audience:Not Specified
Abstract / Description:miRNAs associate with Argonaute (AGO) proteins to silence the expression of mRNA targets by inhibiting translation and promoting deadenylation, decapping, and mRNA degradation. A current model for silencing suggests that AGOs mediate these effects through the sequential recruitment of GW182 proteins, the CCR4-NOT deadenylase complex and the translational repressor and decapping activator DDX6. An alternative model posits that AGOs repress translation by interfering with eIF4A function during 43S ribosomal scanning and that this mechanism is independent of GW182 and the CCR4-NOT complex in Drosophila melanogaster Here, we show that miRNAs, AGOs, GW182, the CCR4-NOT complex, and DDX6/Me31B repress and degrade polyadenylated mRNA targets that are translated via scanning-independent mechanisms in both human and Dm cells. This and additional observations indicate a common mechanism used by these proteins and miRNAs to mediate silencing. This mechanism does not require eIF4A function during ribosomal scanning.
Free Keywords:Ddx6; Gw182; argonaute; deadenylation; eIF4A
External Publication Status:published
Document Type:Article
Communicated by:root
Affiliations:MPI für Entwicklungsbiologie/Abteilung 2 - Biochemie (Elisa Izaurralde)
External Affiliations:Department of Biochemistry, Max Planck Institute for Developmental Biology, Tubingen, Germany elisa.izaurralde@tuebingen.mpg.de.
Identifiers:ISSN:1460-2075 (Electronic) 0261-4189 (Linking) %R 10.1... [ID No:1]
URL:https://www.ncbi.nlm.nih.gov/pubmed/27009120 [ID No:2]
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