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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 4 - Evolutionary Biology (R. Sommer)     Display Documents

ID: 733028.0, MPI für Entwicklungsbiologie / Abteilung 4 - Evolutionary Biology (R. Sommer)
Functional Conservation and Divergence of daf-22 Paralogs in Pristionchus pacificus Dauer Development
Authors:Markov, G. V.; Meyer, J. M.; Panda, O.; Artyukhin, A. B.; Claassen, M.; Witte, H.; Schroeder, F. C.; Sommer, R. J.
Date of Publication (YYYY-MM-DD):2016-10
Title of Journal:Mol Biol Evol
Issue / Number:10
Start Page:2506
End Page:2514
Review Status:Internal review
Audience:Not Specified
Abstract / Description:Small-molecule signaling in nematode dauer formation has emerged as a major model to study chemical communication in development and evolution. Developmental arrest as nonfeeding and stress-resistant dauer larvae represents the major survival and dispersal strategy. Detailed studies in Caenorhabditis elegans and Pristionchus pacificus revealed that small-molecule communication changes rapidly in evolution resulting in extreme structural diversity of small-molecule compounds. In C. elegans, a blend of ascarosides constitutes the dauer pheromone, whereas the P. pacificus dauer pheromone includes additional paratosides and integrates building blocks from diverse primary metabolic pathways. Despite this complexity of small-molecule structures and functions, little is known about the biosynthesis of small molecules in nematodes outside C. elegans Here, we show that the genes encoding enzymes of the peroxisomal beta-oxidation pathway involved in small-molecule biosynthesis evolve rapidly, including gene duplications and domain switching. The thiolase daf-22, the most downstream factor in C. elegans peroxisomal beta-oxidation, has duplicated in P. pacificus, resulting in Ppa-daf-22.1, which still contains the sterol-carrier-protein (SCP) domain that was lost in C. elegans daf-22, and Ppa-daf-22.2. Using the CRISPR/Cas9 system, we induced mutations in both P. pacificus daf-22 genes and identified an unexpected complexity of functional conservation and divergence. Under well-fed conditions, ascaroside biosynthesis proceeds exclusively via Ppa-daf-22.1 In contrast, starvation conditions induce Ppa-daf-22.2 activity, resulting in the production of a specific subset of ascarosides. Gene expression studies indicate a reciprocal up-regulation of both Ppa-daf-22 genes, which is, however, independent of starvation. Thus, our study reveals an unexpected functional complexity of dauer development and evolution.
Free Keywords:C. elegans; Pristionchus pacificus; ascarosides; daf-22; dauer development; phenotypic plasticity; beta-oxidation.
External Publication Status:published
Document Type:Article
Communicated by:MPI für Entwickungsbiologie
Affiliations:MPI für Entwicklungsbiologie/Abteilung 4 - Evolutionsbiologie (Ralf J. Sommer)
External Affiliations:Boyce Thompson Institute, Cornell University Department of Chemistry and Chemical Biology, Cornell University. Boyce Thompson Institute, Cornell University. Max-Planck Institute for Developmental Biology, Spemannstrasse 37, Tubingen, Germany ralf.sommer@tuebingen.mpg.de.
Identifiers:ISSN:1537-1719 (Electronic) 0737-4038 (Linking) %R 10.1... [ID No:1]
URL:https://www.ncbi.nlm.nih.gov/pubmed/27189572 [ID No:2]
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