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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 4 - Evolutionary Biology (R. Sommer)     Display Documents



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ID: 733054.0, MPI für Entwicklungsbiologie / Abteilung 4 - Evolutionary Biology (R. Sommer)
Assaying Predatory Feeding Behaviors in Pristionchus and Other Nematodes
Authors:Lightfoot, J. W.; Wilecki, M.; Okumura, M.; Sommer, R. J.
Date of Publication (YYYY-MM-DD):2016-09-04
Title of Journal:J Vis Exp
Issue / Number:115
Sequence Number of Article:54404
Review Status:Internal review
Audience:Not Specified
Abstract / Description:This protocol provides multiple methods for the analysis and quantification of predatory feeding behaviors in nematodes. Many nematode species including Pristionchus pacificus display complex behaviors, the most striking of which is the predation of other nematode larvae. However, as these behaviors are absent in the model organism Caenorhabditis elegans, they have thus far only recently been described in detail along with the development of reliable behavioral assays (1). These predatory behaviors are dependent upon phenotypically plastic but fixed mouth morphs making the correct identification and categorization of these animals essential. In P. pacificus there are two mouth types, the stenostomatous and eurystomatous morphs (2), with only the wide mouthed eurystomatous containing an extra tooth and being capable of killing other nematode larvae. Through the isolation of an abundance of size matched prey larvae and subsequent exposure to predatory nematodes, assays including both "corpse assays" and "bite assays" on correctly identified mouth morph nematodes are possible. These assays provide a means to rapidly quantify predation success rates and provide a detailed behavioral analysis of individual nematodes engaged in predatory feeding activities. In addition, with the use of a high-speed camera, visualization of changes in pharyngeal activity including tooth and pumping dynamics are also possible.
External Publication Status:published
Document Type:Article
Communicated by:MPI für Entwickungsbiologie
Affiliations:MPI für Entwicklungsbiologie/Abteilung 4 - Evolutionsbiologie (Ralf J. Sommer)
External Affiliations:Department for Evolutionary Biology, Max-Planck Institute for Developmental Biology; ralf.sommer@tuebingen.mpg.de.
Identifiers:ISSN:1940-087X (Electronic) 1940-087X (Linking) %R 10.3... [ID No:1]
URL:https://www.ncbi.nlm.nih.gov/pubmed/27684744 [ID No:2]
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